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Open AccessCommunication

Ectopic Expression of Cold Responsive LlaCIPK Gene Enhances Cold Stress Tolerance in Nicotiana tabacum

1
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
Directorate of Onion and Garlic, Rajgurunagar 410505, India
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Department of Biotechnology, Kumaon University Bhimtal Campus, Bhimtal 263136, India
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Defence Institute of Bio-Energy Research, Goraparao, Haldwani 263139, India
5
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Lab of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning 530004, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Genes 2019, 10(6), 446; https://doi.org/10.3390/genes10060446
Received: 22 April 2019 / Revised: 31 May 2019 / Accepted: 31 May 2019 / Published: 12 June 2019
(This article belongs to the Section Plant Genetics and Genomics)
Low-temperature stress severely affects the growth, development, and geographical distribution of various crop plants, resulting in significant economic loss to producers. In a quest to identify cold-regulated genes, we constructed a cDNA suppression subtractive library from a high altitude adapted ecotype of Lepidium. We cloned a cold-induced gene LlaCIPK from the subtracted cDNA library which gave homology to Arabidopsis CIPK15 gene. The predicted 3D structure of LlaCIPK protein also showed homology with Arabidopsis CIPK protein. Quantitative real-time PCR analysis in Lepidium seedlings exposed to 6 h of cold stress shows a 3-fold increase in the expression of LlaCIPK transcript. The expression of LlaCIPK was also differentially regulated by ethylene, CaCl2, ABA, and SA treatments. Ethylene and CaCl2 treatments up regulated LlaCIPK expression, whereas ABA and SA treatments down regulated the LlaCIPK expression. Transgenic plants overexpressing LlaCIPK gene under constitutive promoter show an increased level of proline and cell membrane stability. Taken together, our results suggest that the LlaCIPK contributes to the cold-response pathway in Lepidium plants. View Full-Text
Keywords: cold; CIPK; gene discovery; Lepidium; abiotic stress cold; CIPK; gene discovery; Lepidium; abiotic stress
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Aslam, M.; Fakher, B.; Anandhan, S.; Pande, V.; Ahmed, Z.; Qin, Y. Ectopic Expression of Cold Responsive LlaCIPK Gene Enhances Cold Stress Tolerance in Nicotiana tabacum. Genes 2019, 10, 446.

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