Next Article in Journal
Genomic Evidence of Recombination in the Basidiomycete Wallemia mellicola
Previous Article in Journal
Analysis of the Full-Length Pyriform Spidroin Gene Sequence
Open AccessCommunication

De Novo Sequencing, Assembly, and Annotation of Four Threespine Stickleback Genomes Based on Microfluidic Partitioned DNA Libraries

Department of Environmental Sciences, Zoology, University of Basel, Vesalgasse 1, CH-4051 Basel, Switzerland
Biodiversity Research Centre and Zoology Department, University of British Columbia, Vancouver, BC V6T 1Z4, Canada
Institute of Ecology and Evolution, University of Bern, Baltzerstrasse 6, CH-3012 Bern, Switzerland
Canada’s Michael Smith Genome Sciences Centre at BC Cancer, Vancouver, BC V5Z 4S6, Canada
Department of Medical Genetics, University of British Columbia, Vancouver, BC V6T 1Z3, Canada
Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada
Faculty of Biological & Environmental Sciences, University of Helsinki, Viikinkaari 1, FI-00014 Helsinki, Finland
Author to whom correspondence should be addressed.
Genes 2019, 10(6), 426;
Received: 13 March 2019 / Revised: 24 May 2019 / Accepted: 27 May 2019 / Published: 3 June 2019
(This article belongs to the Section Technologies and Resources for Genetics)
The threespine stickleback is a geographically widespread and ecologically highly diverse fish that has emerged as a powerful model system for evolutionary genomics and developmental biology. Investigations in this species currently rely on a single high-quality reference genome, but would benefit from the availability of additional, independently sequenced and assembled genomes. We present here the assembly of four new stickleback genomes, based on the sequencing of microfluidic partitioned DNA libraries. The base pair lengths of the four genomes reach 92–101% of the standard reference genome length. Together with their de novo gene annotation, these assemblies offer a resource enhancing genomic investigations in stickleback. The genomes and their annotations are available from the Dryad Digital Repository ( View Full-Text
Keywords: Gasterosteus aculeatus; genome assembly Gasterosteus aculeatus; genome assembly
Show Figures

Figure 1

MDPI and ACS Style

Berner, D.; Roesti, M.; Bilobram, S.; Chan, S.K.; Kirk, H.; Pandoh, P.; Taylor, G.A.; Zhao, Y.; Jones, S.J.M.; DeFaveri, J. De Novo Sequencing, Assembly, and Annotation of Four Threespine Stickleback Genomes Based on Microfluidic Partitioned DNA Libraries. Genes 2019, 10, 426.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop