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Open AccessArticle

Analysis of miRNAs Targeted Storage Regulatory Genes during Soybean Seed Development Based on Transcriptome Sequencing

1
College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
2
Department of Horticulture, Michigan State University, East Lansing, MI 48824, USA
3
Green Food Research Institute in Heilongjiang Province, Harbin 150030, Heilongjiang, China
4
Department of Plant Soil and Microbe Science, Michigan State University, East Lansing, MI 48824, USA
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Genes 2019, 10(6), 408; https://doi.org/10.3390/genes10060408
Received: 21 April 2019 / Revised: 17 May 2019 / Accepted: 23 May 2019 / Published: 28 May 2019
(This article belongs to the Section Plant Genetics and Genomics)
Soybeans are an important cash crop and are widely used as a source of vegetable protein and edible oil. MicroRNAs (miRNA) are endogenous small RNA that play an important regulatory role in the evolutionarily conserved system of gene expression. In this study, we selected four lines with extreme phenotypes, as well as high or low protein and oil content, from the chromosome segment substitution line (CSSL) constructed from suinong (SN14) and ZYD00006, and planted and sampled at three stages of grain development for small RNA sequencing and expression analysis. The sequencing results revealed the expression pattern of miRNA in the materials, and predicted miRNA-targeted regulatory genes, including 1967 pairs of corresponding relationships between known-miRNA and their target genes, as well as 597 pairs of corresponding relationships between novel-miRNA and their target genes. After screening and annotating genes that were targeted for regulation, five specific genes were identified to be differentially expressed during seed development and subsequently analyzed for their regulatory relationship with miRNAs. The expression pattern of the targeted gene was verified by Real-time Quantitative PCR (RT-qPCR). Our research provides more information about the miRNA regulatory network in soybeans and further identifies useful genes that regulate storage during soy grain development, providing a theoretical basis for the regulation of soybean quality traits. View Full-Text
Keywords: soybean; transcriptome sequencing; miRNA; storage; target genes soybean; transcriptome sequencing; miRNA; storage; target genes
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Yu, J.-Y.; Zhang, Z.-G.; Huang, S.-Y.; Han, X.; Wang, X.-Y.; Pan, W.-J.; Qin, H.-T.; Qi, H.-D.; Yin, Z.-G.; Qu, K.-X.; Zhang, Z.-X.; Liu, S.-S.; Jiang, H.-W.; Liu, C.-Y.; Hu, Z.-B.; Wu, X.-X.; Chen, Q.-S.; Xin, D.-W.; Qi, Z.-M. Analysis of miRNAs Targeted Storage Regulatory Genes during Soybean Seed Development Based on Transcriptome Sequencing. Genes 2019, 10, 408.

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