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Open AccessArticle

Role of Autophagy-Related Gene atg22 in Developmental Process and Virulence of Fusarium oxysporum

1
School of Life Sciences, Chongqing University, Chongqing 401331, China
2
Department of Plant Pathology, University of Poonch AJK, Rawalkot 12350, Pakistan
3
Department of Entomology, University of Poonch AJK, Rawalkot 12350, Pakistan
4
Department of Botany, The University of Azad Jammu & Kashmir, Muzafarabad13100, Pakistan
*
Authors to whom correspondence should be addressed.
Genes 2019, 10(5), 365; https://doi.org/10.3390/genes10050365
Received: 30 March 2019 / Revised: 1 May 2019 / Accepted: 6 May 2019 / Published: 13 May 2019
(This article belongs to the Section Microbial Genetics and Genomics)
Autophagy is a universal catabolic process preserved in eukaryotes from yeast to plants and mammals. The main purpose of autophagy is to degrade cytoplasmic materials within the lysosome/vacuole lumen and generate an internal nutrient pool that is recycled back to the cytosol during nutrient stress. Here, Fusarium oxysporum was utilized as a model organism, and we found that autophagy assumes an imperative job in affecting the morphology, development, improvement and pathogenicity of F. oxysporum. The search of autophagy pathway components from the F. oxysporum genome database recognized putative orthologs of 16 core autophagy-related (ATG) genes of yeast, which additionally incorporate the ubiquitin-like protein atg22. Present study elucidates the unreported role of Foatg22 in formation of autophagosomes. The deletion mutant of Foatg22 did not demonstrate positive monodansylcadaverine (MDC) staining, which exposed that Foatg22 is required for autophagy in F. oxysporum. Moreover, the ∆Foatg22 strains exhibited a decrease in hyphal development and conidiation, and reduction in pathogenicity on potato tubers and leaves of potato plant. The hyphae of ∆Foatg22 mutants were less dense when contrasted with wild-type (WT) and overexpression (OE) mutants. Our perceptions demonstrated that Foatg22 might be a key regulator for the control of dry rot disease in tuber and root crops during postharvest stage. View Full-Text
Keywords: Fusarium oxysporum; autophagy; potato dry rot; filamentous fungi; virulence Fusarium oxysporum; autophagy; potato dry rot; filamentous fungi; virulence
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Khalid, A.R.; Zhang, S.; Luo, X.; Mehmood, K.; Rahim, J.; Shaheen, H.; Dong, P.; Qiu, D.; Ren, M. Role of Autophagy-Related Gene atg22 in Developmental Process and Virulence of Fusarium oxysporum. Genes 2019, 10, 365.

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