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Genes 2019, 10(4), 251; https://doi.org/10.3390/genes10040251

The Transcriptome of Verticillium dahliae Responds Differentially Depending on the Disease Susceptibility Level of the Olive (Olea europaea L.) Cultivar

1
Center for Advanced Studies in Olive Grove and Olive Oils, Department of Experimental Biology, University of Jaén, 23071 Jaén, Spain
2
Department of Crop Protection, Institute for Sustainable Agriculture, Agencia Estatal Consejo Superior de Investigaciones Científicas (CSIC), Campus ‘Alameda del Obispo’, Avenida Menéndez Pidal s/n, 14004 Córdoba, Spain
*
Author to whom correspondence should be addressed.
Received: 22 February 2019 / Revised: 22 March 2019 / Accepted: 22 March 2019 / Published: 27 March 2019
(This article belongs to the Section Microbial Genetics and Genomics)
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Abstract

Among biotic constraints affecting olive trees cultivation worldwide, the soil-borne fungus Verticillium dahliae is considered one of the most serious threats. Olive cultivars display differential susceptibility to the disease, but our knowledge on the pathogen’s responses when infecting varieties differing in susceptibility is scarce. A comparative transcriptomic analysis (RNA-seq) was conducted in olive cultivars Picual (susceptible) and Frantoio (tolerant). RNA samples originated from roots during the first two weeks after inoculation with V. dahliae defoliating (D) pathotype. Verticillium dahliae mRNA amount was overwhelmingly higher in roots of the susceptible cultivar, indicating that proliferation of pathogen biomass is favored in ‘Picual’. A significant larger number of V. dahliae unigenes (11 fold) were only induced in this cultivar. Seven clusters of differentially expressed genes (DEG) were identified according to time-course expression patterns. Unigenes potentially coding for niche-adaptation, pathogenicity, virulence and microsclerotia development were induced in ‘Picual’, while in ‘Frantoio’ expression remained negligible or null. Verticillium dahliae D pathotype transcriptome responses are qualitatively and quantitatively different, and depend on cultivar susceptibility level. The much larger V. dahliae biomass found in ‘Picual’ roots is a consequence of both host and pathogen DEG explaining, to a large extent, the higher aggressiveness exerted over this cultivar. View Full-Text
Keywords: defoliating pathotype; effector; pathogenicity; RNA-seq; susceptibility; vascular pathogen; Verticillium dahliae transcriptome; Verticillium wilt of olive defoliating pathotype; effector; pathogenicity; RNA-seq; susceptibility; vascular pathogen; Verticillium dahliae transcriptome; Verticillium wilt of olive
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Jiménez-Ruiz, J.; Leyva-Pérez, M.O.; Gómez-Lama Cabanás, C.; Barroso, J.B.; Luque, F.; Mercado-Blanco, J. The Transcriptome of Verticillium dahliae Responds Differentially Depending on the Disease Susceptibility Level of the Olive (Olea europaea L.) Cultivar. Genes 2019, 10, 251.

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