Next Article in Journal
The Interaction between Sex and Hyperlipidemia on Gout Risk Is Modulated by HLA-B Polymorphic Variants in Adult Taiwanese
Next Article in Special Issue
Simplified Transformation of Ostreococcus tauri Using Polyethylene Glycol
Previous Article in Journal
MYC Oncogene Contributions to Release of Cell Cycle Brakes
Open AccessArticle

Sequencing and Phylogenetic Analysis of Chloroplast Genes in Freshwater Raphidophytes

Laboratoire d’Océanologie et des Geosciences, UMR CNRS 8187, Université du Littoral Côte d’Opale, 62930 Wimereux, France
Aquatic Ecology, Department of Biology, Lund University, 22362 Lund, Sweden
Author to whom correspondence should be addressed.
Genes 2019, 10(3), 245;
Received: 8 February 2019 / Revised: 19 March 2019 / Accepted: 20 March 2019 / Published: 22 March 2019
(This article belongs to the Special Issue Genetics and Genomics of Phytoplankton)
The complex evolution of chloroplasts in microalgae has resulted in highly diverse pigment profiles. Freshwater raphidophytes, for example, display a very different pigment composition to marine raphidophytes. To investigate potential differences in the evolutionary origin of chloroplasts in these two groups of raphidophytes, the plastid genomes of the freshwater species Gonyostomum semen and Vacuolaria virescens were sequenced. To exclusively sequence the organelle genomes, chloroplasts were manually isolated and amplified using single-cell whole-genome-amplification. Assembled and annotated chloroplast genes of the two species were phylogenetically compared to the marine raphidophyte Heterosigma akashiwo and other evolutionarily more diverse microalgae. These phylogenetic comparisons confirmed the high relatedness of all investigated raphidophyte species despite their large differences in pigment composition. Notable differences regarding the presence of light-independent protochlorophyllide oxidoreductase (LIPOR) genes among raphidophyte algae were also revealed in this study. The whole-genome amplification approach proved to be useful for isolation of chloroplast DNA from nuclear DNA. Although only approximately 50% of the genomes were covered, this was sufficient for a multiple gene phylogeny representing large parts of the chloroplast genes. View Full-Text
Keywords: raphidophytes; chloroplast genome; whole-genome-amplification; phylogeny; LIPOR raphidophytes; chloroplast genome; whole-genome-amplification; phylogeny; LIPOR
Show Figures

Figure 1

MDPI and ACS Style

Sassenhagen, I.; Rengefors, K. Sequencing and Phylogenetic Analysis of Chloroplast Genes in Freshwater Raphidophytes. Genes 2019, 10, 245.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop