Next Article in Journal
The Interaction between Sex and Hyperlipidemia on Gout Risk Is Modulated by HLA-B Polymorphic Variants in Adult Taiwanese
Previous Article in Journal
MYC Oncogene Contributions to Release of Cell Cycle Brakes
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessArticle
Genes 2019, 10(3), 245; https://doi.org/10.3390/genes10030245

Sequencing and Phylogenetic Analysis of Chloroplast Genes in Freshwater Raphidophytes

1
Laboratoire d’Océanologie et des Geosciences, UMR CNRS 8187, Université du Littoral Côte d’Opale, 62930 Wimereux, France
2
Aquatic Ecology, Department of Biology, Lund University, 22362 Lund, Sweden
*
Author to whom correspondence should be addressed.
Received: 8 February 2019 / Revised: 19 March 2019 / Accepted: 20 March 2019 / Published: 22 March 2019
(This article belongs to the Special Issue Genetics and Genomics of Phytoplankton)
  |  
PDF [1429 KB, uploaded 28 March 2019]
  |     |  

Abstract

The complex evolution of chloroplasts in microalgae has resulted in highly diverse pigment profiles. Freshwater raphidophytes, for example, display a very different pigment composition to marine raphidophytes. To investigate potential differences in the evolutionary origin of chloroplasts in these two groups of raphidophytes, the plastid genomes of the freshwater species Gonyostomum semen and Vacuolaria virescens were sequenced. To exclusively sequence the organelle genomes, chloroplasts were manually isolated and amplified using single-cell whole-genome-amplification. Assembled and annotated chloroplast genes of the two species were phylogenetically compared to the marine raphidophyte Heterosigma akashiwo and other evolutionarily more diverse microalgae. These phylogenetic comparisons confirmed the high relatedness of all investigated raphidophyte species despite their large differences in pigment composition. Notable differences regarding the presence of light-independent protochlorophyllide oxidoreductase (LIPOR) genes among raphidophyte algae were also revealed in this study. The whole-genome amplification approach proved to be useful for isolation of chloroplast DNA from nuclear DNA. Although only approximately 50% of the genomes were covered, this was sufficient for a multiple gene phylogeny representing large parts of the chloroplast genes. View Full-Text
Keywords: raphidophytes; chloroplast genome; whole-genome-amplification; phylogeny; LIPOR raphidophytes; chloroplast genome; whole-genome-amplification; phylogeny; LIPOR
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Sassenhagen, I.; Rengefors, K. Sequencing and Phylogenetic Analysis of Chloroplast Genes in Freshwater Raphidophytes. Genes 2019, 10, 245.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top