Next Article in Journal
Challenges in the Integration of Omics and Non-Omics Data
Previous Article in Journal
A Comparison Between Two Assays for Measuring Seminal Oxidative Stress and their Relationship with Sperm DNA Fragmentation and Semen Parameters
Previous Article in Special Issue
Dynamic Expression of Interferon Lambda Regulated Genes in Primary Fibroblasts and Immune Organs of the Chicken
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessArticle

Chicken Embryonic-Stem Cells Are Permissive to Poxvirus Recombinant Vaccine Vectors

1
Section of Virology, Department of Medicine, St Mary’s Campus, Imperial College London, Norfolk Place, London W2 1PG, UK
2
Univ Lyon, Université Lyon 1, INSERM, INRA, Stem Cell and Brain Research Institute, U1208, USC1361 Bron, France
*
Authors to whom correspondence should be addressed.
Genes 2019, 10(3), 237; https://doi.org/10.3390/genes10030237
Received: 18 February 2019 / Revised: 14 March 2019 / Accepted: 15 March 2019 / Published: 20 March 2019
(This article belongs to the Special Issue Genomics of Avian Viral Infections)
  |  
PDF [3534 KB, uploaded 27 March 2019]
  |  

Abstract

The discovery of mammalian pluripotent embryonic stem cells (ESC) has revolutionised cell research and regenerative medicine. More recently discovered chicken ESC (cESC), though less intensively studied, are increasingly popular as vaccine substrates due to a dearth of avian cell lines. Information on the comparative performance of cESC with common vaccine viruses is limited. Using RNA-sequencing, we compared cESC transcriptional programmes elicited by stimulation with chicken type I interferon or infection with vaccine viruses routinely propagated in primary chicken embryo fibroblasts (CEF). We used poxviruses (fowlpox virus (FWPV) FP9, canarypox virus (CNPV), and modified vaccinia virus Ankara (MVA)) and a birnavirus (infectious bursal disease virus (IBDV) PBG98). Interferon-stimulated genes (ISGs) were induced in cESC to levels comparable to those in CEF and immortalised chicken fibroblast DF-1 cells. cESC are permissive (with distinct host transcriptional responses) to MVA, FP9, and CNPV but, surprisingly, not to PBG98. MVA, CNPV, and FP9 suppressed innate immune responses, while PBG98 induced a subset of ISGs. Dysregulation of signalling pathways (i.e., NFκB, TRAF) was observed, which might affect immune responses and viral replication. In conclusion, we show that cESC are an attractive alternative substrate to study and propagate poxvirus recombinant vaccine vectors. View Full-Text
Keywords: embryonic stem cells; RNA-sequencing; pluripotency; recombinant vaccine viruses embryonic stem cells; RNA-sequencing; pluripotency; recombinant vaccine viruses
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Giotis, E.S.; Montillet, G.; Pain, B.; Skinner, M.A. Chicken Embryonic-Stem Cells Are Permissive to Poxvirus Recombinant Vaccine Vectors. Genes 2019, 10, 237.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top