Next Article in Journal
Cell-Selective Regulation of CFTR Gene Expression: Relevance to Gene Editing Therapeutics
Next Article in Special Issue
Food Tracking Perspective: DNA Metabarcoding to Identify Plant Composition in Complex and Processed Food Products
Previous Article in Journal
The Patchy Distribution of Restriction–Modification System Genes and the Conservation of Orphan Methyltransferases in Halobacteria
Previous Article in Special Issue
Tubulin-Based DNA Barcode: Principle and Applications to Complex Food Matrices
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessArticle
Genes 2019, 10(3), 234; https://doi.org/10.3390/genes10030234

Development of a DNA Barcoding-Like Approach to Detect Mustard Allergens in Wheat Flours

1
FEM2-Ambiente, Piazza della Scienza 2, I-20126 Milano, Italy
2
BARILLA G. e R. FRATELLI Spa, Via Mantova, 166-43122 Parma, Italy
3
Zooplantlab, Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, I-20126 Milano, Italy
*
Author to whom correspondence should be addressed.
Received: 8 February 2019 / Revised: 2 March 2019 / Accepted: 14 March 2019 / Published: 19 March 2019
(This article belongs to the Special Issue DNA Barcoding and Metabarcoding of Complex Matrices)
  |  
PDF [911 KB, uploaded 27 March 2019]
  |  

Abstract

The spread of food allergens is a topic of global importance due to its impact on public health. National and International regulations ask food producers and manufacturers to declare product compositions on the label, especially in case of processed raw materials. Wheat flour (Triticum aestivum) can be contaminated by a wide range of species belonging to the Brassicaceae in the field or during grain harvests, storage, and processing. Among them, mustards (Brassica nigra, Brassica juncea and Sinapis alba) are well known allergenic species. Often, food quality laboratories adopt an ELISA approach to detect the presence of mustard species. However, this approach shows cross-reactivity with other non-allergenic species such as Brassica napus (rapeseed). In the last few years, DNA barcoding was proposed as a valid identification method, and it is now commonly used in the authentication of food products. This study aims to set up an easy and rapid DNA-based tool to detect mustard allergenic species. DNA barcoding (matK and ITS2) and chromosome markers (A6, B, C1 genome regions) were selected, and specific primers were validated on incurred reference food matrices. The developed test was proven to be able to distinguish mustard from rapeseed and wheat, overcoming cross-reactivity with Brassica napus. View Full-Text
Keywords: allergen detection; DNA barcoding; Brassicaceae; Brassica napus; processed food; Triticum aestivum allergen detection; DNA barcoding; Brassicaceae; Brassica napus; processed food; Triticum aestivum
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Frigerio, J.; Pellesi, R.; Mezzasalma, V.; De Mattia, F.; Galimberti, A.; Lambertini, F.; Suman, M.; Zanardi, S.; Leporati, A.; Labra, M. Development of a DNA Barcoding-Like Approach to Detect Mustard Allergens in Wheat Flours. Genes 2019, 10, 234.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top