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Genes 2019, 10(3), 231; https://doi.org/10.3390/genes10030231

H3K27me3 Depletion during Differentiation Promotes Myogenic Transcription in Porcine Satellite Cells

1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2,* and 1,2,*
1
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction (Huazhong Agricultural University), Ministry of Education, Wuhan 430070, China
2
The Cooperative Innovation Center for Sustainable Pig Production—Swine Breeding and Reproduction Innovation Platform, Huazhong Agricultural University, Wuhan 430070, China
*
Authors to whom correspondence should be addressed.
Received: 26 January 2019 / Revised: 23 February 2019 / Accepted: 11 March 2019 / Published: 19 March 2019
(This article belongs to the Section Molecular Genetics and Genomics)
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Abstract

Background: Porcine skeletal muscle satellite cells play important roles in myogenesis and muscle regeneration. Integrated analysis of transcriptome and histone modifications would reveal epigenomic roles in promoting myogenic differentiation in swine. Methods: Porcine satellite cells (PSCs) were isolated and in-vitro cultured from newborn piglets. RNA Sequencing (RNA-Seq) and Chromatin Immunoprecipitation Sequencing (ChIP-Seq) experiments were performed using proliferating cells and terminal myotubes in order to interrogate the transcriptomic profiles, as well as the distribution of histone markers—H3K4me3, H3K27me3, and H3K27ac—and RNA polymerase II. Results: The study identified 917 differentially expressed genes during cell differentiation. The landscape of epigenetic marks was displayed on a genome-wide scale, which had globally shrunken. H3K27me3 reinforcement participated in obstructing the transcription of proliferation-related genes, while its depletion was closely related to the up-regulation of myogenic genes. Furthermore, the degree of H3K27me3 modification was dramatically reduced by 50%, and 139 myogenic genes were upregulated to promote cell differentiation. Conclusions: The depletion of H3K27me3 was shown to promote porcine satellite cell differentiation through upregulating the transcription level of myogenic genes. Our findings in this study provide new insights of the epigenomic mechanisms occurring during myogenic differentiation, and shed light on chromatin states and the dynamics underlying myogenesis. View Full-Text
Keywords: pig; H3K27me3; porcine satellite cells; differentiation; skeletal muscle pig; H3K27me3; porcine satellite cells; differentiation; skeletal muscle
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Wang, S.; Sun, Y.; Ren, R.; Xie, J.; Tian, X.; Zhao, S.; Li, X.; Cao, J. H3K27me3 Depletion during Differentiation Promotes Myogenic Transcription in Porcine Satellite Cells. Genes 2019, 10, 231.

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