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Genes 2019, 10(3), 221; https://doi.org/10.3390/genes10030221

DNA Replication Timing Enters the Single-Cell Era

Laboratory for Developmental Epigenetics, RIKEN Center for Biosystems Dynamics Research (BDR), Kobe, Hyogo 650-0047, Japan
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Received: 7 February 2019 / Revised: 12 March 2019 / Accepted: 12 March 2019 / Published: 15 March 2019
(This article belongs to the Special Issue DNA Replication Timing: Where, When, How and Why?)
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Abstract

In mammalian cells, DNA replication timing is controlled at the level of megabase (Mb)-sized chromosomal domains and correlates well with transcription, chromatin structure, and three-dimensional (3D) genome organization. Because of these properties, DNA replication timing is an excellent entry point to explore genome regulation at various levels and a variety of studies have been carried out over the years. However, DNA replication timing studies traditionally required at least tens of thousands of cells, and it was unclear whether the replication domains detected by cell population analyses were preserved at the single-cell level. Recently, single-cell DNA replication profiling methods became available, which revealed that the Mb-sized replication domains detected by cell population analyses were actually well preserved in individual cells. In this article, we provide a brief overview of our current knowledge on DNA replication timing regulation in mammals based on cell population studies, outline the findings from single-cell DNA replication profiling, and discuss future directions and challenges. View Full-Text
Keywords: DNA replication timing; replication domain; mammalian chromosome; 3D genome organization; single-cell Repli-seq (scRepli-seq) DNA replication timing; replication domain; mammalian chromosome; 3D genome organization; single-cell Repli-seq (scRepli-seq)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Hiratani, I.; Takahashi, S. DNA Replication Timing Enters the Single-Cell Era. Genes 2019, 10, 221.

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