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Article

Overexpression of AtWRKY30 Transcription Factor Enhances Heat and Drought Stress Tolerance in Wheat (Triticum aestivum L.)

1
Botany Department, Faculty of Science, Tanta University, Tanta 31527, Egypt
2
Botany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
3
Timber Trees Research Department, Sabahia Horticulture Research Station, Horticulture Research Institute, Agriculture Research Center, Alexandria 21526, Egypt
4
UMR CNRS 8256 (B2A), Université Paris VI, 75005 Paris, France
5
Department of Biology, Xavier University, Cincinnati, OH 45207, USA
*
Author to whom correspondence should be addressed.
Genes 2019, 10(2), 163; https://doi.org/10.3390/genes10020163
Received: 12 December 2018 / Revised: 21 January 2019 / Accepted: 23 January 2019 / Published: 20 February 2019
(This article belongs to the Section Plant Genetics and Genomics)
Drought and heat factors have negative impacts on wheat yield and growth worldwide. Improving wheat tolerance to heat and drought stress is of the utmost importance to maintain crop yield. WRKY transcription factors help improve plant resistance to environmental factors. In this investigation, Arabidopsis WRKY30 (AtWRKY30) transcription factor was cloned and expressed in wheat. Plants growth, biomass, gas-exchange attributes, chlorophyll content, relative water content, prolines content, soluble proteins content, soluble sugars content, and antioxidant enzymes activities (catalase (CAT), superoxide dismutase (SOD), peroxidase (POX), and ascorbate peroxidase (APX)) of the AtWRKY30-overexpressing wheat plants were higher than those of the wild type. However, levels of electrolyte leakage, malondialdehyde, and hydrogen peroxide of the AtWRKY30-overexpressing wheat plants were significantly less than those of the wild-type. Additionally, the expression level of antioxidant enzyme-encoding genes and stress-responsive genes (ERF5a, DREB1, DREB3, WRKY19, TIP2, and AQP7) were significantly induced in the transgenic wheat plants in comparison with the wild type. In conclusion, the results demonstrated that AtWRKY30 overexpression promotes heat and drought tolerance in wheat by inducing gas-exchange attributes, antioxidant machinery, osmolytes biosynthesis, and stress-related gene expression. AtWRKY30 could serve as a potential candidate gene for improving stress tolerance in wheat. View Full-Text
Keywords: wheat; AtWRKY30; heat; drought; antioxidant machinery; gene expression wheat; AtWRKY30; heat; drought; antioxidant machinery; gene expression
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MDPI and ACS Style

El-Esawi, M.A.; Al-Ghamdi, A.A.; Ali, H.M.; Ahmad, M. Overexpression of AtWRKY30 Transcription Factor Enhances Heat and Drought Stress Tolerance in Wheat (Triticum aestivum L.). Genes 2019, 10, 163. https://doi.org/10.3390/genes10020163

AMA Style

El-Esawi MA, Al-Ghamdi AA, Ali HM, Ahmad M. Overexpression of AtWRKY30 Transcription Factor Enhances Heat and Drought Stress Tolerance in Wheat (Triticum aestivum L.). Genes. 2019; 10(2):163. https://doi.org/10.3390/genes10020163

Chicago/Turabian Style

El-Esawi, Mohamed A., Abdullah A. Al-Ghamdi, Hayssam M. Ali, and Margaret Ahmad. 2019. "Overexpression of AtWRKY30 Transcription Factor Enhances Heat and Drought Stress Tolerance in Wheat (Triticum aestivum L.)" Genes 10, no. 2: 163. https://doi.org/10.3390/genes10020163

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