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Article

Overexpression of StDREB2 Transcription Factor Enhances Drought Stress Tolerance in Cotton (Gossypium barbadense L.)

1
Botany Department, Faculty of Science, Tanta University, Tanta 31527, Egypt
2
Biological Sciences Department, Faculty of Science, University of Jeddah, Jeddah 21577, Saudi Arabia
*
Author to whom correspondence should be addressed.
Genes 2019, 10(2), 142; https://doi.org/10.3390/genes10020142
Received: 12 December 2018 / Revised: 17 January 2019 / Accepted: 21 January 2019 / Published: 14 February 2019
(This article belongs to the Section Plant Genetics and Genomics)
Drought stress significantly restricts plant growth and crop productivity. Cotton is the most important textile fiber and oilseed crop worldwide, and its cultivation is affected by drought stress, particularly in dry regions. Improving cotton tolerance to drought stress using the advanced genetic engineering technologies is a promising strategy to maintain crop production and fiber quality and meet the increasing worldwide fiber and oil demand. Dehydration-responsive element binding (DREB) transcription factors play a main role in regulating stresses-tolerance pathways in plant. This study investigated whether potato DREB2 (StDREB2) overexpression can improve drought tolerance in cotton. StDREB2 transcription factor was isolated and overexpressed in cotton. Plant biomass, boll number, relative water content, soluble sugars content, soluble protein content, chlorophyll content, proline content, gas-exchange parameters, and antioxidants enzymes (POD, CAT, SOD, GST) activity of the StDREB2-overexpressing cotton plants were higher than those of wild type plants. By contrast, the contents of malondialdehyde, hydrogen peroxide and superoxide anion of StDREB2-overexpressing transgenic plants were significantly lower than that of the wild type plants. Moreover, the transgenic cotton lines revealed higher expression levels of antioxidant genes (SOD, CAT, POD, GST) and stress-tolerant genes (GhERF2, GhNAC3, GhRD22, GhDREB1A, GhDREB1B, GhDREB1C) compared to wild-type plants. Taken together, these findings showed that StDREB2 overexpression augments drought stress tolerance in cotton by inducing plant biomass, gas-exchange characteristics, reactive oxygen species (ROS) scavenging, antioxidant enzymes activities, osmolytes accumulation, and expression of stress-related genes. As a result, StDREB2 could be an important candidate gene for drought-tolerant cotton breeding. View Full-Text
Keywords: StDREB2; transgenic cotton; drought; antioxidants; osmolytes; genes expression StDREB2; transgenic cotton; drought; antioxidants; osmolytes; genes expression
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MDPI and ACS Style

El-Esawi, M.A.; Alayafi, A.A. Overexpression of StDREB2 Transcription Factor Enhances Drought Stress Tolerance in Cotton (Gossypium barbadense L.). Genes 2019, 10, 142. https://doi.org/10.3390/genes10020142

AMA Style

El-Esawi MA, Alayafi AA. Overexpression of StDREB2 Transcription Factor Enhances Drought Stress Tolerance in Cotton (Gossypium barbadense L.). Genes. 2019; 10(2):142. https://doi.org/10.3390/genes10020142

Chicago/Turabian Style

El-Esawi, Mohamed A., and Aisha A. Alayafi 2019. "Overexpression of StDREB2 Transcription Factor Enhances Drought Stress Tolerance in Cotton (Gossypium barbadense L.)" Genes 10, no. 2: 142. https://doi.org/10.3390/genes10020142

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