Dual Oncogenic/Anti-Oncogenic Role of PATZ1 in FRTL5 Rat Thyroid Cells Transformed by the Ha-RasV12 Oncogene
Abstract
:1. Introduction
2. Materials and Methods
2.1 FRTL5-Ha-RasV12 Cell Model and its Derivative FRTL5-Ras-PATZ1
2.2. Tumor Engraftment in Nude Mice
2.3. Histopathological Analysis and Immunohistochemistry
2.4. Terminal Deoxynucleotidyl Transferase Mediated Deoxy Uridine Triphosphate Nick End Labeling Assay
2.5. Spheroid-Forming and Self-Renewal Assays
2.6. RNA Extraction and qRT-PCR
2.7. Statistical Analysis
3. Results
3.1. PATZ1 Expression Enhances Tumor Onset of FRTL5-Ras Mouse Xenograft Models
3.2. PATZ1 Expression Enhances Self-Renewal Ability of Thyroid Cancer Stem-Like Cells
3.3. Thyrosphere Diameter is Decreased in PATZ1-Expressing FRTL5-Ras Cells
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Vitiello, M.; Palma, G.; Monaco, M.; Bello, A.M.; Camorani, S.; Francesca, P.; Rea, D.; Barbieri, A.; Chiappetta, G.; Vita, G.D.; et al. Dual Oncogenic/Anti-Oncogenic Role of PATZ1 in FRTL5 Rat Thyroid Cells Transformed by the Ha-RasV12 Oncogene. Genes 2019, 10, 127. https://doi.org/10.3390/genes10020127
Vitiello M, Palma G, Monaco M, Bello AM, Camorani S, Francesca P, Rea D, Barbieri A, Chiappetta G, Vita GD, et al. Dual Oncogenic/Anti-Oncogenic Role of PATZ1 in FRTL5 Rat Thyroid Cells Transformed by the Ha-RasV12 Oncogene. Genes. 2019; 10(2):127. https://doi.org/10.3390/genes10020127
Chicago/Turabian StyleVitiello, Michela, Giuseppe Palma, Mario Monaco, Anna Maria Bello, Simona Camorani, Paola Francesca, Domenica Rea, Antonio Barbieri, Gennaro Chiappetta, Gabriella De Vita, and et al. 2019. "Dual Oncogenic/Anti-Oncogenic Role of PATZ1 in FRTL5 Rat Thyroid Cells Transformed by the Ha-RasV12 Oncogene" Genes 10, no. 2: 127. https://doi.org/10.3390/genes10020127
APA StyleVitiello, M., Palma, G., Monaco, M., Bello, A. M., Camorani, S., Francesca, P., Rea, D., Barbieri, A., Chiappetta, G., Vita, G. D., Cerchia, L., Arra, C., & Fedele, M. (2019). Dual Oncogenic/Anti-Oncogenic Role of PATZ1 in FRTL5 Rat Thyroid Cells Transformed by the Ha-RasV12 Oncogene. Genes, 10(2), 127. https://doi.org/10.3390/genes10020127