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Article

CaMad2 Promotes Multiple Aspects of Genome Stability Beyond Its Direct Function in Chromosome Segregation

Department of Biology, Gustavus Adolphus College, Saint Peter, MN 56082, USA
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Author to whom correspondence should be addressed.
Genes 2019, 10(12), 1013; https://doi.org/10.3390/genes10121013
Received: 18 November 2019 / Revised: 29 November 2019 / Accepted: 2 December 2019 / Published: 5 December 2019
(This article belongs to the Special Issue Genome Plasticity of Human and Plant Pathogenic Fungi)
Mad2 is a central component of the spindle assembly checkpoint required for accurate chromosome segregation. Additionally, in some organisms, Mad2 has roles in preventing mutations and recombination through the DNA damage response. In the fungal pathogen Candida albicans, CaMad2 has previously been shown to be required for accurate chromosome segregation, survival in high levels of hydrogen peroxide, and virulence in a mouse model of infection. In this work, we showed that CaMad2 promotes genome stability through its well-characterized role in promoting accurate chromosome segregation and through reducing smaller scale chromosome changes due to recombination and DNA damage repair. Deletion of MAD2 decreased cell growth, increased marker loss rates, increased sensitivity to microtubule-destabilizing drugs, and increased sensitivity to DNA damage inducing treatments. CaMad2-GFP localized to dots, consistent with a role in kinetochore binding, and to the nuclear periphery, consistent with an additional role in DNA damage. Furthermore, deletion of MAD2 increases growth on fluconazole, and fluconazole treatment elevates whole chromosome loss rates in the mad2∆/∆ strain, suggesting that CaMad2 may be important for preventing fluconazole resistance via aneuploidy. View Full-Text
Keywords: Mad2; spindle assembly checkpoint; genome instability; Candida albicans; fluconazole Mad2; spindle assembly checkpoint; genome instability; Candida albicans; fluconazole
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MDPI and ACS Style

Vossen, M.L.; Alhosawi, H.M.; Aney, K.J.; Burrack, L.S. CaMad2 Promotes Multiple Aspects of Genome Stability Beyond Its Direct Function in Chromosome Segregation. Genes 2019, 10, 1013. https://doi.org/10.3390/genes10121013

AMA Style

Vossen ML, Alhosawi HM, Aney KJ, Burrack LS. CaMad2 Promotes Multiple Aspects of Genome Stability Beyond Its Direct Function in Chromosome Segregation. Genes. 2019; 10(12):1013. https://doi.org/10.3390/genes10121013

Chicago/Turabian Style

Vossen, Maicy L., Hanaa M. Alhosawi, Katherine J. Aney, and Laura S. Burrack 2019. "CaMad2 Promotes Multiple Aspects of Genome Stability Beyond Its Direct Function in Chromosome Segregation" Genes 10, no. 12: 1013. https://doi.org/10.3390/genes10121013

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