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The Role of Insulation in Patterning Gene Expression

Center for Integrative Genomics (CIG), University of Lausanne, Genopode Building, 1015 Lausanne, Switzerland
Author to whom correspondence should be addressed.
Genes 2019, 10(10), 767;
Received: 2 September 2019 / Revised: 19 September 2019 / Accepted: 24 September 2019 / Published: 28 September 2019
(This article belongs to the Special Issue Transcriptional Regulation of Early Embryogenesis)
Development is orchestrated by regulatory elements that turn genes ON or OFF in precise spatial and temporal patterns. Many safety mechanisms prevent inappropriate action of a regulatory element on the wrong gene promoter. In flies and mammals, dedicated DNA elements (insulators) recruit protein factors (insulator binding proteins, or IBPs) to shield promoters from regulatory elements. In mammals, a single IBP called CCCTC-binding factor (CTCF) is known, whereas genetic and biochemical analyses in Drosophila have identified a larger repertoire of IBPs. How insulators function at the molecular level is not fully understood, but it is currently thought that they fold chromosomes into conformations that affect regulatory element-promoter communication. Here, we review the discovery of insulators and describe their properties. We discuss recent genetic studies in flies and mice to address the question: Is gene insulation important for animal development? Comparing and contrasting observations in these two species reveal that they have different requirements for insulation, but that insulation is a conserved and critical gene regulation strategy. View Full-Text
Keywords: insulator; gene insulation; IBP; CTCF; gene regulation; genome topology; Drosophila; mouse insulator; gene insulation; IBP; CTCF; gene regulation; genome topology; Drosophila; mouse
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MDPI and ACS Style

Özdemir, I.; Gambetta, M.C. The Role of Insulation in Patterning Gene Expression. Genes 2019, 10, 767.

AMA Style

Özdemir I, Gambetta MC. The Role of Insulation in Patterning Gene Expression. Genes. 2019; 10(10):767.

Chicago/Turabian Style

Özdemir, Isa, and Maria C. Gambetta. 2019. "The Role of Insulation in Patterning Gene Expression" Genes 10, no. 10: 767.

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