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Open AccessArticle

Coordinated Activity of Transcriptional Networks Responding to the Pattern of Action Potential Firing in Neurons

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Personalized Genomics Laboratory, Center for Computational Systems Biology, Prairie View A&M University, Prairie View, TX 77446, USA
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DP Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
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Department of Pathology, New York Medical College, Valhalla, NY 10595, USA
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Section on Nervous System Development and Plasticity, the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), NIH, Bethesda, MD 20892, USA
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Division of Medical Imaging Products, U.S. Food and Drug Administration, Silver Spring, 20993 MD, USA
*
Author to whom correspondence should be addressed.
Genes 2019, 10(10), 754; https://doi.org/10.3390/genes10100754
Received: 4 September 2019 / Revised: 17 September 2019 / Accepted: 20 September 2019 / Published: 26 September 2019
Transcriptional responses to the appropriate temporal pattern of action potential firing are essential for long-term adaption of neuronal properties to the functional activity of neural circuits and environmental experience. However, standard transcriptome analysis methods can be too limited in identifying critical aspects that coordinate temporal coding of action potential firing with transcriptome response. A Pearson correlation analysis was applied to determine how pairs of genes in the mouse dorsal root ganglion (DRG) neurons are coordinately expressed in response to stimulation producing the same number of action potentials by two different temporal patterns. Analysis of 4728 distinct gene-pairs related to calcium signaling, 435,711 pairs of transcription factors, 820 pairs of voltage-gated ion channels, and 86,862 pairs of calcium signaling genes with transcription factors indicated that genes become coordinately activated by distinct action potential firing patterns and this depends on the duration of stimulation. Moreover, a measure of expression variance revealed that the control of transcripts abundances is sensitive to the pattern of stimulation. Thus, action potentials impact intracellular signaling and the transcriptome in dynamic manner that not only alter gene expression levels significantly (as previously reported) but also affects the control of their expression fluctuations and profoundly remodel the transcriptional networks. View Full-Text
Keywords: calcium voltage-dependent channels; calcium signaling; covariance analysis; DRG neurons; gene expression variability; potassium voltage-gated channels; sodium voltage-gated channels; transcription factors calcium voltage-dependent channels; calcium signaling; covariance analysis; DRG neurons; gene expression variability; potassium voltage-gated channels; sodium voltage-gated channels; transcription factors
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Iacobas, D.A.; Iacobas, S.; Lee, P.R.; Cohen, J.E.; Fields, R.D. Coordinated Activity of Transcriptional Networks Responding to the Pattern of Action Potential Firing in Neurons. Genes 2019, 10, 754.

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