Effects of Fat and Fatty Acids on the Formation of Autolysosomes in the Livers from Yellow Catfish Pelteobagrus Fulvidraco
Abstract
:1. Introduction
2. Materials and Methods
2.1. Identification and Tissue Distribution of Gene Expression
2.2. Feed Preparation and Fish Culture
2.3. In Vitro Studies
2.3.1. Primary Hepatocytes Culture and Treatments
2.3.2. Cell Viability and Non-Esterified Fatty Acid (NEFA) Concentrations Detection
2.3.3. Flow Cytometry and the Laser Confocal Microscope for Detecting the Formation of Autolysosomes
2.3.4. Real-Time Quantitative Polymerase Chain Reaction (qPCR)
2.4. Statistical Analysis
3. Results
3.1. Molecular Characterization
3.2. Tissue Distribution of Gene Expression
3.3. Transcriptional Responses of Nine Autolysosome-Related Genes by High-Fat Diet
3.4. Effects of Palmitic Acid (PA) Incubations on the Formation of Autolysosomes
3.5. Effects of Oleic Acid (OA) Incubations on the Formation of Autolysosomes
4. Discussion
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Gene | Accession No. | 5′ Untranslated Regions (UTR)(bp) | ORF (bp) | 3′ UTR (bp) | Full Length (bp) | Protein (aa) |
---|---|---|---|---|---|---|
vps11 | MH301091 | 22 | 2784 | 274 | 3080 | 928 |
vps16 | MH301092 | 48 | 2508 | 589 | 3144 | 835 |
vps18 | MH301093 | 305 | 2979 | 98 | 3379 | 992 |
vps33b | MH301094 | 69 | 1853 | 230 | 2230 | 617 |
vps41 | MH301095 | 67 | 2564 | 427 | 2991 | 854 |
lamp1 | MH301096 | 84 | 1245 | 167 | 1496 | 415 |
mcoln1 | MH301097 | 217 | 1745 | 196 | 2158 | 581 |
ctsd1 | MH301098 | 86 | 1190 | 569 | 1845 | 396 |
tfeb | MH459004 | 181 | 1475 | 367 | 2023 | 491 |
Genes | Ictalurus punctatus | Danio rerio | Xenopus tropicalis | Rattus norvegicus | Homo sapiens |
---|---|---|---|---|---|
vps11 | 95.1 | 89.3 | 84.1 | 84.6 | 84.4 |
vps16 | 94.73 | 85.87 | 62.80 | 65.67 | 67.71 |
vps18 | 92.04 | 85.99 | 70.67 | 63.61 | 64.61 |
vps33b | 93.19 | 88.01 | 75.12 | 74.64 | 63.65 |
vps41 | 96.02 | 91.80 | 77.52 | 80.09 | 80.56 |
lamp1 | 76.68 | 86.8 | 27.61 | 42.61 | 42.24 |
mcoln1 | 87.44 | 79.9 | 65.82 | 60.91 | 60.90 |
ctsd1 | 92.93 | 85.93 | 63.26 | 63.83 | 67.08 |
tfeb | 86.60 | 69.82 | 44.20 | 48.65 | 46.55 |
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Wu, L.-X.; Wei, C.-C.; Yang, S.-B.; Zhao, T.; Luo, Z. Effects of Fat and Fatty Acids on the Formation of Autolysosomes in the Livers from Yellow Catfish Pelteobagrus Fulvidraco. Genes 2019, 10, 751. https://doi.org/10.3390/genes10100751
Wu L-X, Wei C-C, Yang S-B, Zhao T, Luo Z. Effects of Fat and Fatty Acids on the Formation of Autolysosomes in the Livers from Yellow Catfish Pelteobagrus Fulvidraco. Genes. 2019; 10(10):751. https://doi.org/10.3390/genes10100751
Chicago/Turabian StyleWu, Li-Xiang, Chuan-Chuan Wei, Shui-Bo Yang, Tao Zhao, and Zhi Luo. 2019. "Effects of Fat and Fatty Acids on the Formation of Autolysosomes in the Livers from Yellow Catfish Pelteobagrus Fulvidraco" Genes 10, no. 10: 751. https://doi.org/10.3390/genes10100751