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Open AccessArticle

Differential Gene Expression in Articular Cartilage and Subchondral Bone of Neonatal and Adult Horses

1
Department of Veterinary Clinical Medicine, University of Illinois College of Veterinary Medicine, Urbana, IL 61802, USA
2
Roy J. Carver Biotechnology Center, University of Illinois, Urbana, IL 61801, USA
3
Veterinary Population Medicine Department, University of Minnesota College of Veterinary Medicine, St. Paul, MN 55108, USA
*
Author to whom correspondence should be addressed.
Genes 2019, 10(10), 745; https://doi.org/10.3390/genes10100745
Received: 5 August 2019 / Revised: 11 September 2019 / Accepted: 21 September 2019 / Published: 25 September 2019
(This article belongs to the Special Issue Equine Genetics)
Skeletogenesis is complex and incompletely understood. Derangement of this process likely underlies developmental skeletal pathologies. Examination of tissue-specific gene expression may help elucidate novel skeletal developmental pathways that could contribute to disease risk. Our aim was to identify and functionally annotate differentially expressed genes in equine neonatal and adult articular cartilage (AC) and subchondral bone (SCB). RNA was sequenced from healthy AC and SCB from the fetlock, hock, and stifle joints of 6 foals (≤4 weeks of age) and six adults (8–12 years of age). There was distinct clustering by age and tissue type. After differential expression analysis, functional annotation and pathway analysis were performed using PANTHER and Reactome. Approximately 1115 and 3574 genes were differentially expressed between age groups in AC and SCB, respectively, falling within dozens of overrepresented gene ontology terms and enriched pathways reflecting a state of growth, high metabolic activity, and tissue turnover in the foals. Enriched pathways were dominated by those related to extracellular matrix organization and turnover, and cell cycle and signal transduction. Additionally, we identified enriched pathways related to neural development and neurotransmission in AC and innate immunity in SCB. These represent novel potential mechanisms for disease that can be explored in future work. View Full-Text
Keywords: transcriptome; skeletal development; joints; developmental orthopedic disease; equine transcriptome; skeletal development; joints; developmental orthopedic disease; equine
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MDPI and ACS Style

Kemper, A.M.; Drnevich, J.; McCue, M.E.; McCoy, A.M. Differential Gene Expression in Articular Cartilage and Subchondral Bone of Neonatal and Adult Horses. Genes 2019, 10, 745.

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