Next Article in Journal
Increased Expression of Lipid Metabolism Genes in Early Stages of Wooden Breast Links Myopathy of Broilers to Metabolic Syndrome in Humans
Next Article in Special Issue
Coding RNA Sequencing of Equine Endometrium during Maternal Recognition of Pregnancy
Previous Article in Journal
The Prevalence and Clinical Characteristics of TECTA-Associated Autosomal Dominant Hearing Loss
Previous Article in Special Issue
Previously Identified Genetic Variants in ADGRL3 Are not Associated with Risk for Equine Degenerative Myeloencephalopathy across Breeds
Open AccessArticle

Differential Gene Expression in Articular Cartilage and Subchondral Bone of Neonatal and Adult Horses

Department of Veterinary Clinical Medicine, University of Illinois College of Veterinary Medicine, Urbana, IL 61802, USA
Roy J. Carver Biotechnology Center, University of Illinois, Urbana, IL 61801, USA
Veterinary Population Medicine Department, University of Minnesota College of Veterinary Medicine, St. Paul, MN 55108, USA
Author to whom correspondence should be addressed.
Genes 2019, 10(10), 745;
Received: 5 August 2019 / Revised: 11 September 2019 / Accepted: 21 September 2019 / Published: 25 September 2019
(This article belongs to the Special Issue Equine Genetics)
Skeletogenesis is complex and incompletely understood. Derangement of this process likely underlies developmental skeletal pathologies. Examination of tissue-specific gene expression may help elucidate novel skeletal developmental pathways that could contribute to disease risk. Our aim was to identify and functionally annotate differentially expressed genes in equine neonatal and adult articular cartilage (AC) and subchondral bone (SCB). RNA was sequenced from healthy AC and SCB from the fetlock, hock, and stifle joints of 6 foals (≤4 weeks of age) and six adults (8–12 years of age). There was distinct clustering by age and tissue type. After differential expression analysis, functional annotation and pathway analysis were performed using PANTHER and Reactome. Approximately 1115 and 3574 genes were differentially expressed between age groups in AC and SCB, respectively, falling within dozens of overrepresented gene ontology terms and enriched pathways reflecting a state of growth, high metabolic activity, and tissue turnover in the foals. Enriched pathways were dominated by those related to extracellular matrix organization and turnover, and cell cycle and signal transduction. Additionally, we identified enriched pathways related to neural development and neurotransmission in AC and innate immunity in SCB. These represent novel potential mechanisms for disease that can be explored in future work. View Full-Text
Keywords: transcriptome; skeletal development; joints; developmental orthopedic disease; equine transcriptome; skeletal development; joints; developmental orthopedic disease; equine
Show Figures

Graphical abstract

MDPI and ACS Style

Kemper, A.M.; Drnevich, J.; McCue, M.E.; McCoy, A.M. Differential Gene Expression in Articular Cartilage and Subchondral Bone of Neonatal and Adult Horses. Genes 2019, 10, 745.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop