Human Amnion Epithelial Cells Impair T Cell Proliferation: The Role of HLA-G and HLA-E Molecules
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Preparations
2.2. Cell Culture
2.3. Isolation of EV from hAEC
2.4. Inhibition of EV Release
2.5. Flow Cytometry
2.6. ELISA
2.7. Cell Proliferation Assay
2.8. Gene Profiling by qPCR
2.9. Statistical Analysis
3. Results
3.1. Human Amnion Epithelial Cells Express and Release HLA-Ib Molecules
3.2. hAEC-Derived EV Expressed HLA-G and Epithelial Markers
3.3. HLA-G is Involved in hAEC-Mediated Inhibition of Allogeneic T Cell Proliferation
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Morandi, F.; Marimpietri, D.; Görgens, A.; Gallo, A.; Srinivasan, R.C.; El-Andaloussi, S.; Gramignoli, R. Human Amnion Epithelial Cells Impair T Cell Proliferation: The Role of HLA-G and HLA-E Molecules. Cells 2020, 9, 2123. https://doi.org/10.3390/cells9092123
Morandi F, Marimpietri D, Görgens A, Gallo A, Srinivasan RC, El-Andaloussi S, Gramignoli R. Human Amnion Epithelial Cells Impair T Cell Proliferation: The Role of HLA-G and HLA-E Molecules. Cells. 2020; 9(9):2123. https://doi.org/10.3390/cells9092123
Chicago/Turabian StyleMorandi, Fabio, Danilo Marimpietri, Andre Görgens, Alessia Gallo, Raghuraman Chittor Srinivasan, Samir El-Andaloussi, and Roberto Gramignoli. 2020. "Human Amnion Epithelial Cells Impair T Cell Proliferation: The Role of HLA-G and HLA-E Molecules" Cells 9, no. 9: 2123. https://doi.org/10.3390/cells9092123