Novel Insights into Beta 2 Adrenergic Receptor Function in the rd10 Model of Retinitis Pigmentosa
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Animals
2.2. Treatments
2.3. Quantitative Real Time PCR
2.4. Western Blotting
2.5. Measurement of NE Levels
2.6. Immunohistochemistry and Quantitative Analysis
2.7. Measurement of Scotopic and Photopic Electroretinogram
2.8. Data Analysis
3. Results
3.1. Sympathetic Overdrive in the rd10 Retina: Desensitization Defects
3.2. HIF-1 Regulation of BAR2 Expression
3.3. HIF-1α Stabilization Partially Prevents Reactive Gliosis, Microglial Activation and Cone Loss
3.4. Effects of HIF-1α Stabilization on ERG
4. Discussion
4.1. Overactivation of the Sympathetic System
4.2. HIF-1α Stabilization Reduces BAR2 Upregulation and Restores the Desensitization Cascade
4.3. HIF-1α Stabilization Restores the Neuroinflammatory Cascade and Prevents Cone Loss
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Cammalleri, M.; Dal Monte, M.; Amato, R.; Lapi, D.; Bagnoli, P. Novel Insights into Beta 2 Adrenergic Receptor Function in the rd10 Model of Retinitis Pigmentosa. Cells 2020, 9, 2060. https://doi.org/10.3390/cells9092060
Cammalleri M, Dal Monte M, Amato R, Lapi D, Bagnoli P. Novel Insights into Beta 2 Adrenergic Receptor Function in the rd10 Model of Retinitis Pigmentosa. Cells. 2020; 9(9):2060. https://doi.org/10.3390/cells9092060
Chicago/Turabian StyleCammalleri, Maurizio, Massimo Dal Monte, Rosario Amato, Dominga Lapi, and Paola Bagnoli. 2020. "Novel Insights into Beta 2 Adrenergic Receptor Function in the rd10 Model of Retinitis Pigmentosa" Cells 9, no. 9: 2060. https://doi.org/10.3390/cells9092060