Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Isolation of Eosinophils and Neutrophils from Blood
2.3. Spectroscopic Measurements
2.3.1. UV−Vis Absorption Spectroscopy
2.3.2. Raman Spectroscopy
2.3.3. Fluorescence Microscopy
2.3.4. Coherent Anti-Stokes Raman Scattering Microscopy (CARS)
3. Results
3.1. Resonance Raman Spectra of EPO and MPO in Solutions
3.2. Electronic and Raman Features of Isolated Eosinophils and Neutrophils
3.3. Fluorescence and Coherent Anti-Stokes Raman Scattering Imaging of Human Eosinophils and Neutrophils
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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532 nm | 633 nm | 785 nm | Assignment of Modes with Their Symmetry |
---|---|---|---|
EPO/MPO | EPO/MPO | EPO/MPO | |
756/756 | 756/746 | 756/746 | ν15 (B1g) |
937/933 | 937/933 | 937/933 | ν46, (Eu) |
1005/1005 | 1005/1005 | 1005/1005 | Phenylalanine |
-/- | 1122/1115 | 1122/1115 | ν22 (A2g) |
1135/1135 | -/- | -/- | ν14 (B1g) |
1177/- | 1177/- | -/1177 | ν30 (B2g) |
-/- | 1216/- | -/- | ν13 (B1g) |
-/- | -/1240 | 1252/1252 | ν42 (Eu) |
1314/1314 | 1314/1314 | -/- | ν21 (A2g) |
-/- | 1341/1336 | 1341/1341-/- | ν41 (Eu)/δs(=CH2) |
1367/1367 | 1367/1361 | -/- | ν4 (A1g) |
1395/1395 | 1395/1395 | -/1395 | ν40 (Eu) |
-/- | -/- | 1453/1453 | CH2/CH3, δs(=CH2) |
-/- | -/1534 | -/1534 | unknown |
1555/1555 | -/- | -/- | ν11 (B1g) |
-/- | 1564/- | -/- | ν19 (A2g) |
1588/1588 | -/1580 | 1580/1588 | ν2 (A1g) |
1610/1610 | 1610/- | 1610/- | ν(C = C) |
1638/1638 | -/1638 | -/- | ν10 (B1g) |
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Dorosz, A.; Grosicki, M.; Dybas, J.; Matuszyk, E.; Rodewald, M.; Meyer, T.; Popp, J.; Malek, K.; Baranska, M. Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy. Cells 2020, 9, 2041. https://doi.org/10.3390/cells9092041
Dorosz A, Grosicki M, Dybas J, Matuszyk E, Rodewald M, Meyer T, Popp J, Malek K, Baranska M. Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy. Cells. 2020; 9(9):2041. https://doi.org/10.3390/cells9092041
Chicago/Turabian StyleDorosz, Aleksandra, Marek Grosicki, Jakub Dybas, Ewelina Matuszyk, Marko Rodewald, Tobias Meyer, Jürgen Popp, Kamilla Malek, and Malgorzata Baranska. 2020. "Eosinophils and Neutrophils—Molecular Differences Revealed by Spontaneous Raman, CARS and Fluorescence Microscopy" Cells 9, no. 9: 2041. https://doi.org/10.3390/cells9092041