Chemical Genetics Approach Identifies Abnormal Inflorescence Meristem 1 as a Putative Target of a Novel Sulfonamide That Protects Catalase2-Deficient Arabidopsis against Photorespiratory Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and Growth Conditions
2.2. Chlorophyll Fluorescence
2.3. Metabolite Profiling
2.4. Transcriptome Profiling
2.4.1. ATH1 Microarray Analysis
2.4.2. RNA Sequencing
2.5. Structure–Activity Relationship Analysis
2.6. Affinity Purification
2.7. Mass Spectrometry and Data Analysis
3. Results
3.1. Two m-Sulfamoyl Benzamides Alleviate the Photorespiratory Phenotype of Arabidopsis cat2-2 Mutants
3.2. Pakerine Treatment Results in Distinct Transcriptome Rearrangement upon Photorespiratory Stress
3.3. Bioactivity of Pakerine in Wild-Type Arabidopsis
3.4. Pakerine Alleviates Dark-Induced Senescence
3.5. Structure–Activity Relationship (SAR) Analysis of Pakerine
3.6. The Peroxisomal Enzyme Abnormal Inflorescence Meristem 1 (AIM1) Is a Putative Target of Pakerine and Is Necessary for Pakerine-Induced Alleviation of Dark-Induced Senescence
3.7. JA Signaling Is Not Required for Pakerine Activity
4. Discussion
4.1. Abnormal Inflorescence Meristem1 Is a Putative Protein Target of Pakerine
4.2. Pakerine Acts Independently of JA Signaling
4.3. SA Accumulation Is a Likely Player in Pakerine Function
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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van der Meer, T.; Verlee, A.; Willems, P.; Impens, F.; Gevaert, K.; Testerink, C.; Stevens, C.V.; Van Breusegem, F.; Kerchev, P. Chemical Genetics Approach Identifies Abnormal Inflorescence Meristem 1 as a Putative Target of a Novel Sulfonamide That Protects Catalase2-Deficient Arabidopsis against Photorespiratory Stress. Cells 2020, 9, 2026. https://doi.org/10.3390/cells9092026
van der Meer T, Verlee A, Willems P, Impens F, Gevaert K, Testerink C, Stevens CV, Van Breusegem F, Kerchev P. Chemical Genetics Approach Identifies Abnormal Inflorescence Meristem 1 as a Putative Target of a Novel Sulfonamide That Protects Catalase2-Deficient Arabidopsis against Photorespiratory Stress. Cells. 2020; 9(9):2026. https://doi.org/10.3390/cells9092026
Chicago/Turabian Stylevan der Meer, Tom, Arno Verlee, Patrick Willems, Francis Impens, Kris Gevaert, Christa Testerink, Christian V. Stevens, Frank Van Breusegem, and Pavel Kerchev. 2020. "Chemical Genetics Approach Identifies Abnormal Inflorescence Meristem 1 as a Putative Target of a Novel Sulfonamide That Protects Catalase2-Deficient Arabidopsis against Photorespiratory Stress" Cells 9, no. 9: 2026. https://doi.org/10.3390/cells9092026