Chemical Genetics Approach Identifies Abnormal Inflorescence Meristem 1 as a Putative Target of a Novel Sulfonamide That Protects Catalase2-Deficient Arabidopsis against Photorespiratory Stress

van der Meer, Tom; Verlee, Arno; Willems, Patrick; Impens, Francis; Gevaert, Kris; Testerink, Christa; Stevens, Christian V.; Van Breusegem, Frank; Kerchev, Pavel

doi:10.3390/cells9092026

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Chemical Genetics Approach Identifies Abnormal Inflorescence Meristem 1 as a Putative Target of a Novel Sulfonamide That Protects Catalase2-Deficient Arabidopsis against Photorespiratory Stress

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VIB Center for Plant Systems Biology, B-9052 Ghent, Belgium

Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium

Department of Green Chemistry and Technology, Faculty of Bioscience Engineering, Ghent University, B-9000 Ghent, Belgium

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VIB Proteomics Core, B-9000 Ghent, Belgium

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Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, B-9000 Ghent, Belgium

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VIB Center for Medical Biotechnology, B-9052 Ghent, Belgium

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Laboratory of Plant Physiology, Plant Sciences Group, Wageningen University and Research, 6708 PB Wageningen, The Netherlands

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Department of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech Republic

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Phytophthora Research Centre, Mendel University in Brno, 61300 Brno, Czech Republic

Authors to whom correspondence should be addressed.

Cells 2020, 9(9), 2026; https://doi.org/10.3390/cells9092026

Received: 24 July 2020 / Revised: 26 August 2020 / Accepted: 31 August 2020 / Published: 2 September 2020

(This article belongs to the Section Plant, Algae and Fungi Cell Biology)

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Abstract

Alterations of hydrogen peroxide (H₂O₂) levels have a profound impact on numerous signaling cascades orchestrating plant growth, development, and stress signaling, including programmed cell death. To expand the repertoire of known molecular mechanisms implicated in H₂O₂ signaling, we performed a forward chemical screen to identify small molecules that could alleviate the photorespiratory-induced cell death phenotype of Arabidopsisthaliana mutants lacking H₂O₂-scavenging capacity by peroxisomal catalase2. Here, we report the characterization of pakerine, an m-sulfamoyl benzamide from the sulfonamide family. Pakerine alleviates the cell death phenotype of cat2 mutants exposed to photorespiration-promoting conditions and delays dark-induced senescence in wild-type Arabidopsis leaves. By using a combination of transcriptomics, metabolomics, and affinity purification, we identified abnormal inflorescence meristem 1 (AIM1) as a putative protein target of pakerine. AIM1 is a 3-hydroxyacyl-CoA dehydrogenase involved in fatty acid β-oxidation that contributes to jasmonic acid (JA) and salicylic acid (SA) biosynthesis. Whereas intact JA biosynthesis was not required for pakerine bioactivity, our results point toward a role for β-oxidation-dependent SA production in the execution of H₂O₂-mediated cell death. View Full-Text

Keywords: H₂O₂ signaling; chemical genetics; catalase2-deficient Arabidopsis; photorespiration; abnormal inflorescence meristem 1

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van der Meer, T.; Verlee, A.; Willems, P.; Impens, F.; Gevaert, K.; Testerink, C.; Stevens, C.V.; Van Breusegem, F.; Kerchev, P. Chemical Genetics Approach Identifies Abnormal Inflorescence Meristem 1 as a Putative Target of a Novel Sulfonamide That Protects Catalase2-Deficient Arabidopsis against Photorespiratory Stress. Cells 2020, 9, 2026. https://doi.org/10.3390/cells9092026

AMA Style

van der Meer T, Verlee A, Willems P, Impens F, Gevaert K, Testerink C, Stevens CV, Van Breusegem F, Kerchev P. Chemical Genetics Approach Identifies Abnormal Inflorescence Meristem 1 as a Putative Target of a Novel Sulfonamide That Protects Catalase2-Deficient Arabidopsis against Photorespiratory Stress. Cells. 2020; 9(9):2026. https://doi.org/10.3390/cells9092026

Chicago/Turabian Style

van der Meer, Tom, Arno Verlee, Patrick Willems, Francis Impens, Kris Gevaert, Christa Testerink, Christian V. Stevens, Frank Van Breusegem, and Pavel Kerchev. 2020. "Chemical Genetics Approach Identifies Abnormal Inflorescence Meristem 1 as a Putative Target of a Novel Sulfonamide That Protects Catalase2-Deficient Arabidopsis against Photorespiratory Stress" Cells 9, no. 9: 2026. https://doi.org/10.3390/cells9092026

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Chemical Genetics Approach Identifies Abnormal Inflorescence Meristem 1 as a Putative Target of a Novel Sulfonamide That Protects Catalase2-Deficient Arabidopsis against Photorespiratory Stress

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