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Article

A Broad-Spectrum Antiviral Peptide Blocks Infection of Viruses by Binding to Phosphatidylserine in the Viral Envelope

1
Department of Medical Microbiology, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
2
Institute of Virology, Technical University and Helmholtzzentrum Munich, 81675 Munich, Germany
3
Membrane Biochemistry and Biophysics, Bijvoet Centre for Biomolecular Research, Utrecht University, 3584 CH Utrecht, The Netherlands
4
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands
5
Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands
6
Institute for Virology, University Hospital Düsseldorf, Heinrich-Heine-University, 40225 Düsseldorf, Germany
7
Institute of Chemistry, Chair of Organic Chemistry II, TU Munich, 85748 Garching, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this paper.
Present address: Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, The Netherlands.
§
These authors contributed equally to this paper.
Cells 2020, 9(9), 1989; https://doi.org/10.3390/cells9091989
Received: 11 August 2020 / Revised: 26 August 2020 / Accepted: 27 August 2020 / Published: 29 August 2020
(This article belongs to the Special Issue Cell Biology of Viral Infections)
The ongoing threat of viral infections and the emergence of antiviral drug resistance warrants a ceaseless search for new antiviral compounds. Broadly-inhibiting compounds that act on elements shared by many viruses are promising antiviral candidates. Here, we identify a peptide derived from the cowpox virus protein CPXV012 as a broad-spectrum antiviral peptide. We found that CPXV012 peptide hampers infection by a multitude of clinically and economically important enveloped viruses, including poxviruses, herpes simplex virus-1, hepatitis B virus, HIV-1, and Rift Valley fever virus. Infections with non-enveloped viruses such as Coxsackie B3 virus and adenovirus are not affected. The results furthermore suggest that viral particles are neutralized by direct interactions with CPXV012 peptide and that this cationic peptide may specifically bind to and disrupt membranes composed of the anionic phospholipid phosphatidylserine, an important component of many viral membranes. The combined results strongly suggest that CPXV012 peptide inhibits virus infections by direct interactions with phosphatidylserine in the viral envelope. These results reiterate the potential of cationic peptides as broadly-acting virus inhibitors. View Full-Text
Keywords: antiviral peptide; enveloped viruses; membrane phosphatidylserine; envelope disruption antiviral peptide; enveloped viruses; membrane phosphatidylserine; envelope disruption
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MDPI and ACS Style

Luteijn, R.D.; Praest, P.; Thiele, F.; Sadasivam, S.M.; Singethan, K.; Drijfhout, J.W.; Bach, C.; de Boer, S.M.; Lebbink, R.J.; Tao, S.; Helfer, M.; Bach, N.C.; Protzer, U.; Costa, A.I.; Killian, J.A.; Drexler, I.; Wiertz, E.J.H.J. A Broad-Spectrum Antiviral Peptide Blocks Infection of Viruses by Binding to Phosphatidylserine in the Viral Envelope. Cells 2020, 9, 1989. https://doi.org/10.3390/cells9091989

AMA Style

Luteijn RD, Praest P, Thiele F, Sadasivam SM, Singethan K, Drijfhout JW, Bach C, de Boer SM, Lebbink RJ, Tao S, Helfer M, Bach NC, Protzer U, Costa AI, Killian JA, Drexler I, Wiertz EJHJ. A Broad-Spectrum Antiviral Peptide Blocks Infection of Viruses by Binding to Phosphatidylserine in the Viral Envelope. Cells. 2020; 9(9):1989. https://doi.org/10.3390/cells9091989

Chicago/Turabian Style

Luteijn, Rutger D., Patrique Praest, Frank Thiele, Saravanan M. Sadasivam, Katrin Singethan, Jan W. Drijfhout, Christian Bach, Steffen M. de Boer, Robert J. Lebbink, Sha Tao, Markus Helfer, Nina C. Bach, Ulrike Protzer, Ana I. Costa, J. A. Killian, Ingo Drexler, and Emmanuel J.H.J. Wiertz 2020. "A Broad-Spectrum Antiviral Peptide Blocks Infection of Viruses by Binding to Phosphatidylserine in the Viral Envelope" Cells 9, no. 9: 1989. https://doi.org/10.3390/cells9091989

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