Modulation of Human Adipose Stem Cells’ Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation and Culture of Human Adipose Stem Cells (ASC)
2.2. Human ASC Characterization
2.3. Isolation of Chicken Embryonic Dorsal Root Ganglia (DRG)
2.4. Quantitative Measurement of NT3 by ELISA
2.5. Experimental Design for the Axonal Outgrowth Assay
2.6. Immunocytochemistry of DRG Cultures
2.7. Quantitative Analysis of Axonal Outgrowth
2.8. Quantitative RT-PCR for Regeneration-Associated Genes (RAG) in DRG
2.9. Phosphoproteomic Analysis
2.10. Data Availability
2.11. Statistical Analysis
3. Results
3.1. Human ASC Characterization
3.2. Distinct Effects of NTF on Axonal Outgrowth
3.3. Conditioned Medium Derived from Stimulated Stem Cells
3.4. NT3 Content
3.5. Transcriptional Analysis of RAG in Treated DRG Explants
3.6. Quantitative Phosphoproteomic Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Group | Axonal Length (μm) | Axonal Area (mm2) |
---|---|---|
NGF | 413 ± 182 | 1.903 ± 1.239 |
GDNF | 405 ± 116 | 1.252 ± 0.486 |
BDNF | 419 ± 73 | 1.566 ± 0.479 |
CNTF | 352 ± 74 | 0.766 ± 0.286 |
NT3 | 463 ± 121 | 1.819 ± 0.700 |
NT4 | 291 ± 51 | 0.674 ± 0.123 |
GM | 282 ± 41 | 0.616 ± 0.125 |
CM-ASC | 354 ± 31 | 0.999 ± 0.296 |
CM-NGF-ASC | 526 ± 87 | 2.460 ± 0.586 |
CM-GDNF-ASC | 505 ± 75 | 1.512 ± 0.334 |
CM-BDNF-ASC | 598 ± 118 | 1.918 ± 0.547 |
CM-CNTF-ASC | 599 ± 58 | 2.032 ± 0.643 |
CM-NT3-ASC | 765 ± 134 | 3.423 ± 0.798 |
CM-NT4-ASC | 522 ± 80 | 1.617 ± 0.427 |
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Prautsch, K.M.; Schmidt, A.; Paradiso, V.; Schaefer, D.J.; Guzman, R.; Kalbermatten, D.F.; Madduri, S. Modulation of Human Adipose Stem Cells’ Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro. Cells 2020, 9, 1939. https://doi.org/10.3390/cells9091939
Prautsch KM, Schmidt A, Paradiso V, Schaefer DJ, Guzman R, Kalbermatten DF, Madduri S. Modulation of Human Adipose Stem Cells’ Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro. Cells. 2020; 9(9):1939. https://doi.org/10.3390/cells9091939
Chicago/Turabian StylePrautsch, Katharina M., Alexander Schmidt, Viola Paradiso, Dirk J. Schaefer, Raphael Guzman, Daniel F. Kalbermatten, and Srinivas Madduri. 2020. "Modulation of Human Adipose Stem Cells’ Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro" Cells 9, no. 9: 1939. https://doi.org/10.3390/cells9091939