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Article

VPS72/YL1-Mediated H2A.Z Deposition Is Required for Nuclear Reassembly after Mitosis

1
Institute of Biochemistry and Molecular Cell Biology, Medical School, RWTH Aachen University, 52074 Aachen, Germany
2
Friedrich Miescher Laboratory of the Max Planck Society, Spemannstrasse 39, 72076 Tübingen, Germany
3
Cellular Dynamics Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosowa, Wako, Saitama 351-0198, Japan
*
Authors to whom correspondence should be addressed.
These authors equally contributed to the work.
Cells 2020, 9(7), 1702; https://doi.org/10.3390/cells9071702
Received: 25 May 2020 / Revised: 8 July 2020 / Accepted: 15 July 2020 / Published: 16 July 2020
(This article belongs to the Special Issue Histone Variants from Structure to Molecular Function)
The eukaryotic nucleus remodels extensively during mitosis. Upon mitotic entry, the nuclear envelope breaks down and chromosomes condense into rod-shaped bodies, which are captured by the spindle apparatus and segregated during anaphase. Through telophase, chromosomes decondense and the nuclear envelope reassembles, leading to a functional interphase nucleus. While the molecular processes occurring in early mitosis are intensively investigated, our knowledge about molecular mechanisms of nuclear reassembly is rather limited. Using cell free and cellular assays, we identify the histone variant H2A.Z and its chaperone VPS72/YL1 as important factors for reassembly of a functional nucleus after mitosis. Live-cell imaging shows that siRNA-mediated downregulation of VPS72 extends the telophase in HeLa cells. In vitro, depletion of VPS72 or H2A.Z results in malformed and nonfunctional nuclei. VPS72 is part of two chromatin-remodeling complexes, SRCAP and EP400. Dissecting the mechanism of nuclear reformation using cell-free assays, we, however, show that VPS72 functions outside of the SRCAP and EP400 remodeling complexes to deposit H2A.Z, which in turn is crucial for formation of a functional nucleus. View Full-Text
Keywords: VPS72; YL-1; vacuolar protein sorting 72 homolog; YL1; CFL1; Swc2; TCFL1; H2A.Z; H2AZ; nuclear envelope; nucleolus; mitotic exit; telophase; nuclear reformation VPS72; YL-1; vacuolar protein sorting 72 homolog; YL1; CFL1; Swc2; TCFL1; H2A.Z; H2AZ; nuclear envelope; nucleolus; mitotic exit; telophase; nuclear reformation
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MDPI and ACS Style

Moreno-Andrés, D.; Yokoyama, H.; Scheufen, A.; Holzer, G.; Lue, H.; Schellhaus, A.K.; Weberruss, M.; Takagi, M.; Antonin, W. VPS72/YL1-Mediated H2A.Z Deposition Is Required for Nuclear Reassembly after Mitosis. Cells 2020, 9, 1702. https://doi.org/10.3390/cells9071702

AMA Style

Moreno-Andrés D, Yokoyama H, Scheufen A, Holzer G, Lue H, Schellhaus AK, Weberruss M, Takagi M, Antonin W. VPS72/YL1-Mediated H2A.Z Deposition Is Required for Nuclear Reassembly after Mitosis. Cells. 2020; 9(7):1702. https://doi.org/10.3390/cells9071702

Chicago/Turabian Style

Moreno-Andrés, Daniel, Hideki Yokoyama, Anja Scheufen, Guillaume Holzer, Hongqi Lue, Anna K. Schellhaus, Marion Weberruss, Masatoshi Takagi, and Wolfram Antonin. 2020. "VPS72/YL1-Mediated H2A.Z Deposition Is Required for Nuclear Reassembly after Mitosis" Cells 9, no. 7: 1702. https://doi.org/10.3390/cells9071702

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