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Open AccessFeature PaperArticle

Chemically Functionalized Water-Soluble Single-Walled Carbon Nanotubes Obstruct Vesicular/Plasmalemmal Recycling in Astrocytes Down-Stream of Calcium Ions

1
Departments of Neurobiology and Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA
2
Department of Biomedical Engineering and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
3
Department of Neuroscience, Center for Brain and Spinal Cord Repair and Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA
4
Departments of Chemistry and Chemical and Environmental Engineering and Center for Nanoscale Science and Engineering, University of California, Riverside, CA 92521, USA
5
Carbon Solutions, Inc., Riverside, CA 92507, USA
*
Author to whom correspondence should be addressed.
Deceased on 21 April 2016.
Cells 2020, 9(7), 1597; https://doi.org/10.3390/cells9071597
Received: 11 June 2020 / Revised: 24 June 2020 / Accepted: 27 June 2020 / Published: 1 July 2020
(This article belongs to the Special Issue Astrocytes in Space and Time)
We used single-walled carbon nanotubes chemically functionalized with polyethylene glycol (SWCNT-PEG) to assess the effects of this nanomaterial on astrocytic endocytosis and exocytosis. We observed that the SWCNT-PEG do not affect the adenosine triphosphate (ATP)-evoked Ca2+ elevations in astrocytes but significantly reduce the Ca2+-dependent glutamate release. There was a significant decrease in the endocytic load of the recycling dye during constitutive and ATP-evoked recycling. Furthermore, SWCNT-PEG hampered ATP-evoked exocytotic release of the loaded recycling dye. Thus, by functionally obstructing evoked vesicular recycling, SWCNT-PEG reduced glutamate release from astrocytes via regulated exocytosis. These effects implicate SWCNT-PEG as a modulator of Ca2+-dependent exocytosis in astrocytes downstream of Ca2+, likely at the level of vesicle fusion with/pinching off the plasma membrane. View Full-Text
Keywords: carbon nanotubes; astrocytes; Ca2+ dynamics; glutamate release; membrane recycling carbon nanotubes; astrocytes; Ca2+ dynamics; glutamate release; membrane recycling
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MDPI and ACS Style

Gottipati, M.K.; Bekyarova, E.; Haddon, R.C.; Parpura, V. Chemically Functionalized Water-Soluble Single-Walled Carbon Nanotubes Obstruct Vesicular/Plasmalemmal Recycling in Astrocytes Down-Stream of Calcium Ions. Cells 2020, 9, 1597.

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