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Volume 9
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10.3390/cells9061447
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Article

Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing

by
Hanseul Park
1,2,
Jaein Shin
1,2,
Hwan Choi
1,2,
Byounggook Cho
1,2 and
Jongpil Kim
1,2,*
1
Department of Biomedical Engineering (BK21 Plus), Dongguk University, Seoul 04620, Korea
2
Laboratory of Cell reprogramming and Gene editing, Department of Chemistry, Dongguk University, Seoul 04620, Korea
*
Author to whom correspondence should be addressed.
Cells 2020, 9(6), 1447; https://doi.org/10.3390/cells9061447
Submission received: 1 May 2020 / Revised: 8 June 2020 / Accepted: 9 June 2020 / Published: 10 June 2020
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Abstract

The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has emerged as a powerful technology, with the potential to generate transgenic animals. Particularly, efficient and precise genetic editing with CRISPR/Cas9 offers immense prospects in various biotechnological applications. Here, we report that the histone deacetylase inhibitor valproic acid (VPA) significantly increases the efficiency of CRISPR/Cas9-mediated gene editing in mouse embryonic stem cells and embryos. This effect may be caused through globally enhanced chromatin accessibility, as indicate by histone hyperacetylation. Taken together, our results suggest that VPA can be used to increase the efficacy of CRISPR/Cas9 in generating transgenic systems.
Keywords: embryo; gene editing; CRISPR/Cas9; valproic acid embryo; gene editing; CRISPR/Cas9; valproic acid

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MDPI and ACS Style

Park, H.; Shin, J.; Choi, H.; Cho, B.; Kim, J. Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing. Cells 2020, 9, 1447. https://doi.org/10.3390/cells9061447

AMA Style

Park H, Shin J, Choi H, Cho B, Kim J. Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing. Cells. 2020; 9(6):1447. https://doi.org/10.3390/cells9061447

Chicago/Turabian Style

Park, Hanseul, Jaein Shin, Hwan Choi, Byounggook Cho, and Jongpil Kim. 2020. "Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing" Cells 9, no. 6: 1447. https://doi.org/10.3390/cells9061447

APA Style

Park, H., Shin, J., Choi, H., Cho, B., & Kim, J. (2020). Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing. Cells, 9(6), 1447. https://doi.org/10.3390/cells9061447

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