Next Article in Journal
Bioactive Lipid Signaling in Cardiovascular Disease, Development, and Regeneration
Next Article in Special Issue
With Age Comes Maturity: Biochemical and Structural Transformation of a Human Centriole in the Making
Previous Article in Journal
Large-Scale Drug Screening in Patient-Derived IDHmut Glioma Stem Cells Identifies Several Efficient Drugs among FDA-Approved Antineoplastic Agents
Previous Article in Special Issue
Centering and Shifting of Centrosomes in Cells
Open AccessReview

A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex

Department of Molecular Biology and Genetics, Koc University, 34450 Istanbul, Turkey
*
Author to whom correspondence should be addressed.
Cells 2020, 9(6), 1390; https://doi.org/10.3390/cells9061390
Received: 2 May 2020 / Revised: 23 May 2020 / Accepted: 27 May 2020 / Published: 3 June 2020
(This article belongs to the Special Issue Centrosome)
The mammalian centrosome/cilium complex is composed of the centrosome, the primary cilium and the centriolar satellites, which together regulate cell polarity, signaling, proliferation and motility in cells and thereby development and homeostasis in organisms. Accordingly, deregulation of its structure and functions is implicated in various human diseases including cancer, developmental disorders and neurodegenerative diseases. To better understand these disease connections, the molecular underpinnings of the assembly, maintenance and dynamic adaptations of the centrosome/cilium complex need to be uncovered with exquisite detail. Application of proximity-based labeling methods to the centrosome/cilium complex generated spatial and temporal interaction maps for its components and provided key insights into these questions. In this review, we first describe the structure and cell cycle-linked regulation of the centrosome/cilium complex. Next, we explain the inherent biochemical and temporal limitations in probing the structure and function of the centrosome/cilium complex and describe how proximity-based labeling approaches have addressed them. Finally, we explore current insights into the knowledge we gained from the proximity mapping studies as it pertains to centrosome and cilium biogenesis and systematic characterization of the centrosome, cilium and centriolar satellite interactomes. View Full-Text
Keywords: centriolar satellites; centrosome; cilia; microtubules; ciliopathies; proximity-labeling; BioID; APEX; TurboID centriolar satellites; centrosome; cilia; microtubules; ciliopathies; proximity-labeling; BioID; APEX; TurboID
Show Figures

Figure 1

MDPI and ACS Style

Arslanhan, M.D.; Gulensoy, D.; Firat-Karalar, E.N. A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex. Cells 2020, 9, 1390. https://doi.org/10.3390/cells9061390

AMA Style

Arslanhan MD, Gulensoy D, Firat-Karalar EN. A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex. Cells. 2020; 9(6):1390. https://doi.org/10.3390/cells9061390

Chicago/Turabian Style

Arslanhan, Melis D.; Gulensoy, Dila; Firat-Karalar, Elif N. 2020. "A Proximity Mapping Journey into the Biology of the Mammalian Centrosome/Cilium Complex" Cells 9, no. 6: 1390. https://doi.org/10.3390/cells9061390

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop