Next Article in Journal
Human Liver Spheroids as a Model to Study Aetiology and Treatment of Hepatic Fibrosis
Next Article in Special Issue
Homing and Engraftment of Intravenously Administered Equine Cord Blood-Derived Multipotent Mesenchymal Stromal Cells to Surgically Created Cutaneous Wound in Horses: A Pilot Project
Previous Article in Journal
Progesterone through Progesterone Receptor B Isoform Promotes Rodent Embryonic Oligodendrogenesis
Previous Article in Special Issue
Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells
Article

A Dense Fibrillar Collagen Scaffold Differentially Modulates Secretory Function of iPSC-Derived Vascular Smooth Muscle Cells to Promote Wound Healing

1
Section of Plastic Surgery, Department of Surgery Yale School of Medicine, Yale University, New Haven, CT 06510, USA
2
Vascular Biology and Therapeutics Program and the Department of Surgery, Yale School of Medicine, Yale University, New Haven, CT 06510, USA
3
Department of Biomedical Engineering, Rutgers University, The State University New Jersey, Piscataway, NJ 08854, USA
*
Authors to whom correspondence should be addressed.
Cells 2020, 9(4), 966; https://doi.org/10.3390/cells9040966
Received: 1 March 2020 / Revised: 6 April 2020 / Accepted: 11 April 2020 / Published: 14 April 2020
(This article belongs to the Special Issue Stem Cell Therapy: Wound Healing and Skin Regeneration)
The application of human-induced pluripotent stem cells (hiPSCs) to generate vascular smooth muscle cells (hiPSC-VSMCs) in abundance is a promising strategy for vascular regeneration. While hiPSC-VSMCs have already been utilized for tissue-engineered vascular grafts and disease modeling, there is a lack of investigations exploring their therapeutic secretory factors. The objective of this manuscript was to understand how the biophysical property of a collagen-based scaffold dictates changes in the secretory function of hiPSC-VSMCs while developing hiPSC-VSMC-based therapy for durable regenerative wound healing. We investigated the effect of collagen fibrillar density (CFD) on hiPSC-VSMC’s paracrine secretion and cytokines via the construction of varying density of collagen scaffolds. Our study demonstrated that CFD is a key scaffold property that modulates the secretory function of hiPSC-VSMCs. This study lays the foundation for developing collagen-based scaffold materials for the delivery of hiPSC-VSMCs to promote regenerative healing through guiding paracrine signaling pathways. View Full-Text
Keywords: induced pluripotent stem cell; vascular smooth muscle cell; wound healing; paracrine factors; angiogenesis; inflammation; collagen; biomaterial induced pluripotent stem cell; vascular smooth muscle cell; wound healing; paracrine factors; angiogenesis; inflammation; collagen; biomaterial
Show Figures

Figure 1

MDPI and ACS Style

Dash, B.C.; Setia, O.; Gorecka, J.; Peyvandi, H.; Duan, K.; Lopes, L.; Nie, J.; Berthiaume, F.; Dardik, A.; Hsia, H.C. A Dense Fibrillar Collagen Scaffold Differentially Modulates Secretory Function of iPSC-Derived Vascular Smooth Muscle Cells to Promote Wound Healing. Cells 2020, 9, 966. https://doi.org/10.3390/cells9040966

AMA Style

Dash BC, Setia O, Gorecka J, Peyvandi H, Duan K, Lopes L, Nie J, Berthiaume F, Dardik A, Hsia HC. A Dense Fibrillar Collagen Scaffold Differentially Modulates Secretory Function of iPSC-Derived Vascular Smooth Muscle Cells to Promote Wound Healing. Cells. 2020; 9(4):966. https://doi.org/10.3390/cells9040966

Chicago/Turabian Style

Dash, Biraja C., Ocean Setia, Jolanta Gorecka, Hassan Peyvandi, Kaiti Duan, Lara Lopes, James Nie, Francois Berthiaume, Alan Dardik, and Henry C. Hsia 2020. "A Dense Fibrillar Collagen Scaffold Differentially Modulates Secretory Function of iPSC-Derived Vascular Smooth Muscle Cells to Promote Wound Healing" Cells 9, no. 4: 966. https://doi.org/10.3390/cells9040966

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop