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Article

Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells

1
Department of In Vitro Toxicology and Dermato-Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, 1090 Brussels, Belgium
2
Department of Clinical Chemistry, Microbiology and Immunology, Faculty of Medicine and Health Sciences, Universiteit Gent, 9000 Ghent, Belgium
3
Laboratory of Experimental Hematology, Jules Bordet Institute, Université Libre de Bruxelles, 1000 Brussels, Belgium
4
Genetics and Immune Cell Therapy Unit, Faculty of Sciences, University Mohammed Premier, 60000 Oujda, Morocco
5
Department of Urology, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, 1090 Brussels, Belgium
6
Laboratory of Clinical Cell Therapy, Jules Bordet Institute, Université Libre de Bruxelles, 1070 Brussels, Belgium
7
Osteoarthritis Research Unit, Department of Medicine, University of Montreal Hospital Research Center (CRCHUM), Montreal, QC H2X 3E4, Canada
*
Author to whom correspondence should be addressed.
These authors have contributed equally to this work.
Cells 2020, 9(4), 914; https://doi.org/10.3390/cells9040914
Received: 2 March 2020 / Revised: 25 March 2020 / Accepted: 4 April 2020 / Published: 8 April 2020
(This article belongs to the Special Issue Stem Cell Therapy: Wound Healing and Skin Regeneration)
Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key candidates for allogeneic cell-based, off-the-shelf therapy. However, potential clinical application of allogeneic SKP requires that these cells retain their therapeutic properties under all circumstances and, in particular, in the presence of an inflammation state. Therefore, in this study, we investigated the impact of pro-inflammatory stimulation on the secretome and immunosuppressive properties of SKP. We demonstrated that pro-inflammatory stimulation of SKP significantly changes their expression and the secretion profile of chemo/cytokines and growth factors. Most importantly, we observed that pro-inflammatory stimulated SKP were still able to suppress the graft-versus-host response when cotransplanted with human PBMC in severe-combined immune deficient (SCID) mice, albeit to a much lesser extent than unstimulated SKP. Altogether, this study demonstrates that an inflammatory microenvironment has a significant impact on the immunological properties of SKP. These alterations need to be taken into account when developing allogeneic SKP-based therapies. View Full-Text
Keywords: immunogenicity; inflammation; adult stem cells; skin stem cells; stem cell-microenvironment interactions; cytokines immunogenicity; inflammation; adult stem cells; skin stem cells; stem cell-microenvironment interactions; cytokines
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MDPI and ACS Style

De Kock, J.; Rodrigues, R.M.; Branson, S.; Verhoye, L.; Colemonts-Vroninks, H.; Rombaut, M.; Boeckmans, J.; Neuckermans, J.; Lequeue, S.; Buyl, K.; Merimi, M.; Agha, D.M.; De Boe, V.; Lagneaux, L.; Meuleman, P.; Vanhaecke, T.; Najar, M. Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells. Cells 2020, 9, 914. https://doi.org/10.3390/cells9040914

AMA Style

De Kock J, Rodrigues RM, Branson S, Verhoye L, Colemonts-Vroninks H, Rombaut M, Boeckmans J, Neuckermans J, Lequeue S, Buyl K, Merimi M, Agha DM, De Boe V, Lagneaux L, Meuleman P, Vanhaecke T, Najar M. Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells. Cells. 2020; 9(4):914. https://doi.org/10.3390/cells9040914

Chicago/Turabian Style

De Kock, Joery, Robim M. Rodrigues, Steven Branson, Lieven Verhoye, Haaike Colemonts-Vroninks, Matthias Rombaut, Joost Boeckmans, Jessie Neuckermans, Sien Lequeue, Karolien Buyl, Makram Merimi, Douaa M. Agha, Veerle De Boe, Laurence Lagneaux, Philip Meuleman, Tamara Vanhaecke, and Mehdi Najar. 2020. "Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells" Cells 9, no. 4: 914. https://doi.org/10.3390/cells9040914

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