Human Platelet Lysate Can Replace Fetal Calf Serum as a Protein Source to Promote Expansion and Osteogenic Differentiation of Human Bone-Marrow-Derived Mesenchymal Stromal Cells
Abstract
1. Introduction
2. Materials and Methods
3. Results
3.1. Population Doublings
3.2. Alkaline Phosphatase Activity
3.3. Alizarin Red Staining
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Group | Protein Source and Concentration in ESM | Protein Source and Concentration in ODM |
---|---|---|
F1 | FCS 10% | FCS 1% |
F10 | FCS 10% | FCS 10% |
H1 | hPL 10% | hPL 1% |
H10 | hPL 10% | hPL 10% |
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Karadjian, M.; Senger, A.-S.; Essers, C.; Wilkesmann, S.; Heller, R.; Fellenberg, J.; Simon, R.; Westhauser, F. Human Platelet Lysate Can Replace Fetal Calf Serum as a Protein Source to Promote Expansion and Osteogenic Differentiation of Human Bone-Marrow-Derived Mesenchymal Stromal Cells. Cells 2020, 9, 918. https://doi.org/10.3390/cells9040918
Karadjian M, Senger A-S, Essers C, Wilkesmann S, Heller R, Fellenberg J, Simon R, Westhauser F. Human Platelet Lysate Can Replace Fetal Calf Serum as a Protein Source to Promote Expansion and Osteogenic Differentiation of Human Bone-Marrow-Derived Mesenchymal Stromal Cells. Cells. 2020; 9(4):918. https://doi.org/10.3390/cells9040918
Chicago/Turabian StyleKaradjian, Maria, Anne-Sophie Senger, Christopher Essers, Sebastian Wilkesmann, Raban Heller, Joerg Fellenberg, Rolf Simon, and Fabian Westhauser. 2020. "Human Platelet Lysate Can Replace Fetal Calf Serum as a Protein Source to Promote Expansion and Osteogenic Differentiation of Human Bone-Marrow-Derived Mesenchymal Stromal Cells" Cells 9, no. 4: 918. https://doi.org/10.3390/cells9040918
APA StyleKaradjian, M., Senger, A.-S., Essers, C., Wilkesmann, S., Heller, R., Fellenberg, J., Simon, R., & Westhauser, F. (2020). Human Platelet Lysate Can Replace Fetal Calf Serum as a Protein Source to Promote Expansion and Osteogenic Differentiation of Human Bone-Marrow-Derived Mesenchymal Stromal Cells. Cells, 9(4), 918. https://doi.org/10.3390/cells9040918