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Open AccessArticle

Spatial Expression Pattern of the Major Ca2+-Buffer Proteins in Mouse Retinal Ganglion Cells

1
János Szentágothai Research Centre, University of Pécs, 7624 Pécs, Hungary
2
Retinal Electrical Synapses Research Group, National Brain Research Program (NAP 2.0), Hungarian Academy of Sciences, 1051 Budapest, Hungary
3
Department of Experimental Zoology and Neurobiology, University of Pécs, 7624 Pécs, Hungary
4
Medical School, University of Pécs, 7624 Pécs, Hungary
*
Author to whom correspondence should be addressed.
Contributed equally.
Cells 2020, 9(4), 792; https://doi.org/10.3390/cells9040792
Received: 10 February 2020 / Revised: 20 March 2020 / Accepted: 24 March 2020 / Published: 25 March 2020
(This article belongs to the Special Issue Molecular Biology of Retinal Ganglion Cells)
The most prevalent Ca2+-buffer proteins (CaBPs: parvalbumin—PV; calbindin—CaB; calretinin—CaR) are widely expressed by various neurons throughout the brain, including the retinal ganglion cells (RGCs). Even though their retinal expression has been extensively studied, a coherent assessment of topographical variations is missing. To examine this, we performed immunohistochemistry (IHC) in mouse retinas. We found variability in the expression levels and cell numbers for CaR, with stronger and more numerous labels in the dorso-central area. CaBP+ cells contributed to RGCs with all soma sizes, indicating heterogeneity. We separated four to nine RGC clusters in each area based on expression levels and soma sizes. Besides the overall high variety in cluster number and size, the peripheral half of the temporal retina showed the greatest cluster number, indicating a better separation of RGC subtypes there. Multiple labels showed that 39% of the RGCs showed positivity for a single CaBP, 30% expressed two CaBPs, 25% showed no CaBP expression, and 6% expressed all three proteins. Finally, we observed an inverse relation between CaB and CaR expression levels in CaB/CaR dual- and CaB/CaR/PV triple-labeled RGCs, suggesting a mutual complementary function. View Full-Text
Keywords: parvalbumin; calretinin; calbindin; expression; retina; topography; ganglion cell; calcium buffer protein; expression parvalbumin; calretinin; calbindin; expression; retina; topography; ganglion cell; calcium buffer protein; expression
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Kovács-Öller, T.; Szarka, G.; Tengölics, Á.J.; Ganczer, A.; Balogh, B.; Szabó-Meleg, E.; Nyitrai, M.; Völgyi, B. Spatial Expression Pattern of the Major Ca2+-Buffer Proteins in Mouse Retinal Ganglion Cells. Cells 2020, 9, 792.

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