Intravitreal Co-Administration of GDNF and CNTF Confers Synergistic and Long-Lasting Protection against Injury-Induced Cell Death of Retinal Ganglion Cells in Mice †
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Generation of GDNF- and CNTF-Expressing NS Cell Lines
2.3. Optic Nerve Crush, Neural Stem Cell Transplantations and Anterograde Axonal Tracing
2.4. Analyses of Grafted Neural Stem Cells
2.5. Ganglion Cell Survival and Cell Body Size
2.6. Axonal Regeneration and Intraretinal Axon Growth
3. Results
3.1. Lentiviral Vectors and Transgene Expression in Cultured Neural Stem Cells
3.2. Survival, Differentiation and Transgene Expression in Grafted Neural Stem Cells
3.3. Long-Term Survival of Axotomized Ganglion Cells
3.4. Axon Regeneration into the Distal Optic Nerve Stumps
3.5. Intraretinal Growth of Ganglion Cell Axons
3.6. Soma Size of Ganglion Cells
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Dulz, S.; Bassal, M.; Flachsbarth, K.; Riecken, K.; Fehse, B.; Schlichting, S.; Bartsch, S.; Bartsch, U. Intravitreal Co-Administration of GDNF and CNTF Confers Synergistic and Long-Lasting Protection against Injury-Induced Cell Death of Retinal Ganglion Cells in Mice. Cells 2020, 9, 2082. https://doi.org/10.3390/cells9092082
Dulz S, Bassal M, Flachsbarth K, Riecken K, Fehse B, Schlichting S, Bartsch S, Bartsch U. Intravitreal Co-Administration of GDNF and CNTF Confers Synergistic and Long-Lasting Protection against Injury-Induced Cell Death of Retinal Ganglion Cells in Mice. Cells. 2020; 9(9):2082. https://doi.org/10.3390/cells9092082
Chicago/Turabian StyleDulz, Simon, Mahmoud Bassal, Kai Flachsbarth, Kristoffer Riecken, Boris Fehse, Stefanie Schlichting, Susanne Bartsch, and Udo Bartsch. 2020. "Intravitreal Co-Administration of GDNF and CNTF Confers Synergistic and Long-Lasting Protection against Injury-Induced Cell Death of Retinal Ganglion Cells in Mice" Cells 9, no. 9: 2082. https://doi.org/10.3390/cells9092082