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Open AccessArticle

Basal-Type Breast Cancer Stem Cells Over-Express Chromosomal Passenger Complex Proteins

1
Subdirection of Basic Research, Instituto Nacional de Medicina Genomica, Periférico Sur 4809, Col. Arenal Tepepan, Tlalpan, Mexico City 14610, Mexico
2
Present address Health Sciences departament, Universidad Autónoma Metropolitana, San Rafael Atlixco No. 186, Col. Vicentina, Iztapalapa 09340, Mexico
3
Instituto Nacional de Enfermedades Respiratorias. Calzada de Tlalpan 4502, Col. Sección XVI, Tlalpan, Mexico City 14080, Mexico
*
Author to whom correspondence should be addressed.
Cells 2020, 9(3), 709; https://doi.org/10.3390/cells9030709
Received: 13 February 2020 / Revised: 27 February 2020 / Accepted: 29 February 2020 / Published: 13 March 2020
(This article belongs to the Special Issue Molecular and Cellular Mechanisms of Cancers: Breast Cancer)
(1) Aim: In the present paper we analyzed the transcriptome of CSCs (Cancer Stem Cells), in order to find defining molecular processes of breast cancer. (2) Methods: We performed RNA-Seq from CSCs isolated from the basal cell line MDA-MB-468. Enriched processes and networks were studied using the IPA (Ingenuity Pathway Analysis) tool. Validation was performed with qRT-PCR and the analysis of relevant genes was evaluated by overexpression, flow cytometry and in vivo zebrafish studies. Finally, the clinical relevance of these results was assessed using reported cohorts. (3) Results: We found that CSCs presented marked differences from the non-CSCs, including enrichment in transduction cascades related to stemness, cellular growth, proliferation and apoptosis. Interestingly, CSCs overexpressed a module of co-regulated Chromosomal Passenger Proteins including BIRC5 (survivin), INCENP and AURKB. Overexpression of BIRC5 increased the number of CSCs, as assessed by in vitro and in vivo zebrafish xenotransplant analyses. Analysis of previously published cohorts showed that this co-regulated module was not only overexpressed in basal breast tumors but also associated with relapse-free and overall survival in these patients. (4) Conclusions: These results underline the importance of Cancer Stem Cells in breast cancer progression and point toward the possible use of chromosomal passenger proteins as prognostic factors. View Full-Text
Keywords: breast cancer; networks; stem cells breast cancer; networks; stem cells
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Schwarz-Cruz y Celis, A.; Ceballos-Cancino, G.; Vazquez-Santillan, K.; Espinosa, M.; Zampedri, C.; Bahena, I.; Ruiz, V.; Maldonado, V.; Melendez-Zajgla, J. Basal-Type Breast Cancer Stem Cells Over-Express Chromosomal Passenger Complex Proteins. Cells 2020, 9, 709.

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