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Article

Silencing of Euchromatic Transposable Elements as a Consequence of Nuclear Lamina Dysfunction

1
Dipartimento di Farmacia e Biotecnologie, Alma Mater Studiorum Università di Bologna, 40126 Bologna, Italy
2
CNR Institute of Molecular Genetics “Luigi-Luca Cavalli-Sforza”, Unit of Bologna, 40136 Bologna, Italy
3
IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy
*
Author to whom correspondence should be addressed.
Cells 2020, 9(3), 625; https://doi.org/10.3390/cells9030625
Received: 23 January 2020 / Revised: 28 February 2020 / Accepted: 2 March 2020 / Published: 5 March 2020
(This article belongs to the Collection Lamins and Laminopathies)
Transposable elements (TEs) are mobile genomic sequences that are normally repressed to avoid proliferation and genome instability. Gene silencing mechanisms repress TEs by RNA degradation or heterochromatin formation. Heterochromatin maintenance is therefore important to keep TEs silent. Loss of heterochromatic domains has been linked to lamin mutations, which have also been associated with derepression of TEs. In fact, lamins are structural components of the nuclear lamina (NL), which is considered a pivotal structure in the maintenance of heterochromatin domains at the nuclear periphery in a silent state. Here, we show that a lethal phenotype associated with Lamin loss-of-function mutations is influenced by Drosophila gypsy retrotransposons located in euchromatic regions, suggesting that NL dysfunction has also effects on active TEs located in euchromatic loci. In fact, expression analysis of different long terminal repeat (LTR) retrotransposons and of one non-LTR retrotransposon located near active genes shows that Lamin inactivation determines the silencing of euchromatic TEs. Furthermore, we show that the silencing effect on euchromatic TEs spreads to the neighboring genomic regions, with a repressive effect on nearby genes. We propose that NL dysfunction may have opposed regulatory effects on TEs that depend on their localization in active or repressed regions of the genome. View Full-Text
Keywords: nuclear lamins; nuclear envelope; transposons; TE silencing; gene expression; LamDm0; cosuppression nuclear lamins; nuclear envelope; transposons; TE silencing; gene expression; LamDm0; cosuppression
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MDPI and ACS Style

Cavaliere, V.; Lattanzi, G.; Andrenacci, D. Silencing of Euchromatic Transposable Elements as a Consequence of Nuclear Lamina Dysfunction. Cells 2020, 9, 625. https://doi.org/10.3390/cells9030625

AMA Style

Cavaliere V, Lattanzi G, Andrenacci D. Silencing of Euchromatic Transposable Elements as a Consequence of Nuclear Lamina Dysfunction. Cells. 2020; 9(3):625. https://doi.org/10.3390/cells9030625

Chicago/Turabian Style

Cavaliere, Valeria, Giovanna Lattanzi, and Davide Andrenacci. 2020. "Silencing of Euchromatic Transposable Elements as a Consequence of Nuclear Lamina Dysfunction" Cells 9, no. 3: 625. https://doi.org/10.3390/cells9030625

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