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Article

PPE51 Is Involved in the Uptake of Disaccharides by Mycobacterium tuberculosis

1
Institute of Medical Biology, Polish Academy of Sciences, 93-232 Lodz, Poland
2
Biobank Lab, Department of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 90-237 Lodz, Poland
3
Department of Anthropology, Faculty of Biology and Environmental Protection, University of Lodz, 90-237 Lodz, Poland
4
Department of Immunoparasitology, Faculty of Biology and Environmental Protection, University of Lodz, 90-237 Lodz, Poland
5
Department of Pharmaceutical Sciences, Nesbitt School of Pharmacy, Wilkes University, Wilkes-Barre, PA 18766, USA
*
Author to whom correspondence should be addressed.
Cells 2020, 9(3), 603; https://doi.org/10.3390/cells9030603
Received: 24 January 2020 / Revised: 20 February 2020 / Accepted: 28 February 2020 / Published: 3 March 2020
We have recently found that selected thio-disaccharides possess bactericidal effects against Mycobacterium tuberculosis but not against Escherichia coli or Staphylococcus aureus. Here, we selected spontaneous mutants displaying resistance against the investigated thio-glycoside. According to next-generation sequencing, four of six analyzed mutants which were resistant to high concentrations of the tested chemical carried nonsynonymous mutations in the gene encoding the PPE51 protein. The complementation of these mutants with an intact ppe51 gene returned their sensitivity to the wild-type level. The uptake of tritiated thio-glycoside was significantly more abundant in wild-type Mycobacterium tuberculosis compared to the strain carrying the mutated ppe51 gene. The ppe51 mutations or CRISPR-Cas9-mediated downregulation of PPE51 expression affected the growth of mutant strains on minimal media supplemented with disaccharides (maltose or lactose) but not with glycerol or glucose as the sole carbon and energy source. Taking the above into account, we postulate that PPE51 participates in the uptake of disaccharides by tubercle bacilli. View Full-Text
Keywords: PPE51; tuberculosis; disaccharides; thio-sugars; nutrient uptake PPE51; tuberculosis; disaccharides; thio-sugars; nutrient uptake
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MDPI and ACS Style

Korycka-Machała, M.; Pawełczyk, J.; Borówka, P.; Dziadek, B.; Brzostek, A.; Kawka, M.; Bekier, A.; Rykowski, S.; Olejniczak, A.B.; Strapagiel, D.; Witczak, Z.; Dziadek, J. PPE51 Is Involved in the Uptake of Disaccharides by Mycobacterium tuberculosis. Cells 2020, 9, 603. https://doi.org/10.3390/cells9030603

AMA Style

Korycka-Machała M, Pawełczyk J, Borówka P, Dziadek B, Brzostek A, Kawka M, Bekier A, Rykowski S, Olejniczak AB, Strapagiel D, Witczak Z, Dziadek J. PPE51 Is Involved in the Uptake of Disaccharides by Mycobacterium tuberculosis. Cells. 2020; 9(3):603. https://doi.org/10.3390/cells9030603

Chicago/Turabian Style

Korycka-Machała, Małgorzata, Jakub Pawełczyk, Paulina Borówka, Bożena Dziadek, Anna Brzostek, Malwina Kawka, Adrian Bekier, Sebastian Rykowski, Agnieszka B. Olejniczak, Dominik Strapagiel, Zbigniew Witczak, and Jarosław Dziadek. 2020. "PPE51 Is Involved in the Uptake of Disaccharides by Mycobacterium tuberculosis" Cells 9, no. 3: 603. https://doi.org/10.3390/cells9030603

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