The Phenotype and Functional Activity of Mesenchymal Stromal Cells in Pediatric Patients with Non-Malignant Hematological Diseases
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients
2.2. Isolation of Bone Marrow Mononuclear Cells (BM-MNCs)
2.3. Generation of Mesenchymal Stromal Cells (MSCs)
2.4. Estimation of the Frequency of Progenitor Cells for MSCs Using Colony Forming Units-Fibroblast (CFU-F) Assay
2.5. Immunophenotyping of Mesenchymal Stromal Cells (MSC)
2.6. Differentiation Potential of MSCs of Patients with Non-Malignant Hematological Diseases (NMHD)
2.6.1. Differentiation into Osteoblasts
2.6.2. Differentiation into Adipocytes
2.6.3. Differentiation into Chondrocytes
2.7. Mixed Lymphocyte Reaction (MLR)
2.8. Data Analysis
3. Results
3.1. Patients with Thalassemia Contain more BM-MNCs per Milliliter Bone Marrow than Patients with Sickle Cell Disease (SCD) and Control Group
3.2. BM-MNCs of Patients with SCD Generate More CFU-Fs per 1×106 BM-MNCs Compared to Patients with Thalassemia or Healthy Donors
3.3. BM-MNCs of Patients with NMHD Demonstrate a Normal Generation and Proliferation Potential of Mesenchymal Stromal Cells (MSCs)
3.4. Phenotype of Mesenchymal Stromal Cells (MSCs) Generated by BM-MNCs of Patients with Non-Malignant Hematological Diseases (NMHD)
3.5. Trilineage Differentiation Potential of Mesenchymal Stromal Cells (MSCs) of Patients with Non-Malignant Hematological Diseases (NMHD)
3.6. Immunosuppressive Effect of Mesenchymal Stromal Cells (MSCs) of Patients with Non-Malignant Hematological Diseases (NMHD)
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gender | Healthy donors | TM | SCD | SCN-CN |
---|---|---|---|---|
N | 21 | 10 | 9 | 2 |
Female, N (%) | 12 (60) | 5 (50) | 5 (56) | 2 (100) |
Male, N (%) | 9 (40) | 5 (50) | 4 (44) | 0 |
Age in years: median (range) | 12 (3–16) | 10.5 (4–14) | 14 (9–15) | 7–11 |
Patient’s ID. | Disease | Age (Years)/Gender | Osteoblasts | Adipocytes | Chondrocytes |
---|---|---|---|---|---|
1 | TM | 9/f | *** | * | *** |
2 | TM | 5/m | *** | * | *** |
3 | TM | 13/m | ** | * | *** |
4 | TM | 4/f | *** | * | *** |
11 | SCD | 14/m | ** | *** | *** |
12 | SCD | 14/f | *** | ** | *** |
13 | SCD | 15/f | *** | *** | ** |
20 | SCN | 11/f | *** | *** | *** |
21 | CN | 7/f | *** | ** | *** |
Healthy Donors’ ID | |||||
22 | HD | 8/m | ** | *** | *** |
23 | HD | 6/m | *** | *** | *** |
24 | HD | 13/m | *** | *** | *** |
25 | HD | 4/f | * | *** | *** |
32 | HD | 12/f | *** | *** | *** |
33 | HD | 14/f | *** | * | |
34 | HD | 16/m | *** | ** | *** |
35 | HD | 13/m | *** | ** | *** |
41 | HD | 12/f | ** | *** | *** |
42 | HD | 8/f | *** | *** | *** |
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Kuҫi, Z.; Jordan, C.; Wehner, S.; Sörensen, J.; Jarisch, A.; Salzmann-Manrique, E.; Pfeffermann, L.-M.; Klingebiel, T.; Bader, P.; Kuҫi, S. The Phenotype and Functional Activity of Mesenchymal Stromal Cells in Pediatric Patients with Non-Malignant Hematological Diseases. Cells 2020, 9, 431. https://doi.org/10.3390/cells9020431
Kuҫi Z, Jordan C, Wehner S, Sörensen J, Jarisch A, Salzmann-Manrique E, Pfeffermann L-M, Klingebiel T, Bader P, Kuҫi S. The Phenotype and Functional Activity of Mesenchymal Stromal Cells in Pediatric Patients with Non-Malignant Hematological Diseases. Cells. 2020; 9(2):431. https://doi.org/10.3390/cells9020431
Chicago/Turabian StyleKuҫi, Zyrafete, Christiane Jordan, Sibylle Wehner, Jan Sörensen, Andrea Jarisch, Emilia Salzmann-Manrique, Lisa-Marie Pfeffermann, Thomas Klingebiel, Peter Bader, and Selim Kuҫi. 2020. "The Phenotype and Functional Activity of Mesenchymal Stromal Cells in Pediatric Patients with Non-Malignant Hematological Diseases" Cells 9, no. 2: 431. https://doi.org/10.3390/cells9020431