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Open AccessArticle

Red LED Light Acts on the Mitochondrial Electron Chain of Mammalian Sperm via Light-Time Exposure-Dependent Mechanisms

1
Unit of Animal Reproduction, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, Bellaterra, E-08193 Cerdanyola del Vallès, Spain
2
Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, E-17003 Girona, Spain
3
Unit of Cell Biology, Department of Biology, Faculty of Sciences, University of Girona, E-17003 Girona, Spain
4
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Barcelona, E-08028 Barcelona, Spain
*
Authors to whom correspondence should be addressed.
Joint first authors.
Joint senior authors.
Cells 2020, 9(12), 2546; https://doi.org/10.3390/cells9122546
Received: 10 August 2020 / Revised: 19 November 2020 / Accepted: 25 November 2020 / Published: 26 November 2020
This work analyzes the effects of red LED light on mammalian sperm mitochondrial function, using the pig as an animal model. Liquid-stored pig semen was stimulated with red-light for 1, 5 and 10 min in the presence or absence of oligomycin A, a specific inhibitor of mitochondrial ATP synthase, or carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), a specific disruptor of mitochondrial electron chain. Whereas exposure for 1 and 5 min significantly (p < 0.05) decreased total motility and intracellular ATP levels, irradiation for 10 min induced the opposite effect. Oligomycin A abolished the light-effects on intracellular ATP levels, O2 consumption and mitochondrial membrane potential, whereas compared to non-irradiated samples, FCCP significantly (p < 0.05) increased O2 consumption when sperm were irradiated for 1 min. Both oligomycin A and FCCP significantly (p < 0.05) decreased total motility. Red-light increased cytochrome c oxidase activity with a maximal effect after 5 min of irradiation, which was abolished by both oligomycin A and FCCP. In conclusion, red-light modulates sperm mitochondrial function via electron chain activity in an exposition, time-dependent manner. View Full-Text
Keywords: sperm; photobiology; mitochondrial function; mammalian; oligomycin A; FCCP sperm; photobiology; mitochondrial function; mammalian; oligomycin A; FCCP
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MDPI and ACS Style

Blanco-Prieto, O.; Catalán, J.; Trujillo-Rojas, L.; Peña, A.; Rivera del Álamo, M.M.; Llavanera, M.; Bonet, S.; Fernández-Novell, J.M.; Yeste, M.; Rodríguez-Gil, J.E. Red LED Light Acts on the Mitochondrial Electron Chain of Mammalian Sperm via Light-Time Exposure-Dependent Mechanisms. Cells 2020, 9, 2546. https://doi.org/10.3390/cells9122546

AMA Style

Blanco-Prieto O, Catalán J, Trujillo-Rojas L, Peña A, Rivera del Álamo MM, Llavanera M, Bonet S, Fernández-Novell JM, Yeste M, Rodríguez-Gil JE. Red LED Light Acts on the Mitochondrial Electron Chain of Mammalian Sperm via Light-Time Exposure-Dependent Mechanisms. Cells. 2020; 9(12):2546. https://doi.org/10.3390/cells9122546

Chicago/Turabian Style

Blanco-Prieto, Olga; Catalán, Jaime; Trujillo-Rojas, Lina; Peña, Alejandro; Rivera del Álamo, Maria M.; Llavanera, Marc; Bonet, Sergi; Fernández-Novell, Josep M.; Yeste, Marc; Rodríguez-Gil, Joan E. 2020. "Red LED Light Acts on the Mitochondrial Electron Chain of Mammalian Sperm via Light-Time Exposure-Dependent Mechanisms" Cells 9, no. 12: 2546. https://doi.org/10.3390/cells9122546

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