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Article

Angiogenic Activity of Cytochalasin B-Induced Membrane Vesicles of Human Mesenchymal Stem Cells

1
Openlab “Gene and cell technologies”, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Russia
2
M.M. Shemyakin–Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia
3
Faculty of Medicine and Health Sciences, University of Nottingham, Nottingham LE12 5RD, UK
4
Department of Radiation Oncology, College of Medicine, University of Ibadan, Ibadan 200284, Nigeria
5
Department of Microbiology and Immunology, Reno School of Medicine, University of Nevada, Reno, NV 89557, USA
*
Authors to whom correspondence should be addressed.
Cells 2020, 9(1), 95; https://doi.org/10.3390/cells9010095
Received: 15 November 2019 / Revised: 19 December 2019 / Accepted: 27 December 2019 / Published: 30 December 2019
(This article belongs to the Special Issue Extracellular Vesicles: Biogenesis, Cargo and Disease)
The cytochalasin B-induced membrane vesicles (CIMVs) are suggested to be used as a vehicle for the delivery of therapeutics. However, the angiogenic activity and therapeutic potential of human mesenchymal stem/stromal cells (MSCs) derived CIMVs (CIMVs-MSCs) remains unknown. Objectives: The objectives of this study were to analyze the morphology, size distribution, molecular composition, and angiogenic properties of CIMVs-MSCs. Methods: The morphology of CIMVs-MSC was analyzed by scanning electron microscopy. The proteomic analysis, multiplex analysis, and immunostaining were used to characterize the molecular composition of the CIMVs-MSCs. The transfer of surface proteins from a donor to a recipient cell mediated by CIMVs-MSCs was demonstrated using immunostaining and confocal microscopy. The angiogenic potential of CIMVs-MSCs was evaluated using an in vivo approach of subcutaneous implantation of CIMVs-MSCs in mixture with Matrigel matrix. Results: Human CIMVs-MSCs retain parental MSCs content, such as growth factors, cytokines, and chemokines: EGF, FGF-2, Eotaxin, TGF-α, G-CSF, Flt-3L, GM-CSF, Fractalkine, IFNα2, IFN-γ, GRO, IL-10, MCP-3, IL-12p40, MDC, IL-12p70, IL-15, sCD40L, IL-17A, IL-1RA, IL-1a, IL-9, IL-1b, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IP-10, MCP-1, MIP_1a, MIP-1b, TNF-α, TNF-β, VEGF. CIMVs-MSCs also have the expression of surface receptors similar to those in parental human MSCs (CD90+, CD29+, CD44+, CD73+). Additionally, CIMVs-MSCs could transfer membrane receptors to the surfaces of target cells in vitro. Finally, CIMVs-MSCs can induce angiogenesis in vivo after subcutaneous injection into adult rats. Conclusions: Human CIMVs-MSCs have similar content, immunophenotype, and angiogenic activity to those of the parental MSCs. Therefore, we believe that human CIMVs-MSCs could be used for cell free therapy of degenerative diseases. View Full-Text
Keywords: extracellular vesicles; microvesicles; membrane vesicles; cytochalasin B; mesenchymal stem cells; angiogenesis; cell-free therapy extracellular vesicles; microvesicles; membrane vesicles; cytochalasin B; mesenchymal stem cells; angiogenesis; cell-free therapy
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MDPI and ACS Style

Gomzikova, M.O.; Zhuravleva, M.N.; Vorobev, V.V.; Salafutdinov, I.I.; Laikov, A.V.; Kletukhina, S.K.; Martynova, E.V.; Tazetdinova, L.G.; Ntekim, A.I.; Khaiboullina, S.F.; Rizvanov, A.A. Angiogenic Activity of Cytochalasin B-Induced Membrane Vesicles of Human Mesenchymal Stem Cells. Cells 2020, 9, 95. https://doi.org/10.3390/cells9010095

AMA Style

Gomzikova MO, Zhuravleva MN, Vorobev VV, Salafutdinov II, Laikov AV, Kletukhina SK, Martynova EV, Tazetdinova LG, Ntekim AI, Khaiboullina SF, Rizvanov AA. Angiogenic Activity of Cytochalasin B-Induced Membrane Vesicles of Human Mesenchymal Stem Cells. Cells. 2020; 9(1):95. https://doi.org/10.3390/cells9010095

Chicago/Turabian Style

Gomzikova, Marina O., Margarita N. Zhuravleva, Vyacheslav V. Vorobev, Ilnur I. Salafutdinov, Alexander V. Laikov, Sevindzh K. Kletukhina, Ekaterina V. Martynova, Leysan G. Tazetdinova, Atara I. Ntekim, Svetlana F. Khaiboullina, and Albert A. Rizvanov 2020. "Angiogenic Activity of Cytochalasin B-Induced Membrane Vesicles of Human Mesenchymal Stem Cells" Cells 9, no. 1: 95. https://doi.org/10.3390/cells9010095

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