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Open AccessArticle

Spectral Characterization of Stem Cell-Derived Myelination within the Injured Adult PNS Using the Solvatochromic Dye Nile Red

1
Division of Neurosurgery, Department of Surgery, Texas Tech University, Lubbock, TX 79415, USA
2
Hotchkiss Brain Institute, Calgary, AB T2N 4N1, Canada
3
Department of Clinical Neurosciences, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada
4
Department of Comparative Biology and Experimental Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada
5
The Neuro, Montreal Neurological Institute, 3801 University Drive, Montreal, QC H3A 2B4, Canada
*
Author to whom correspondence should be addressed.
Cells 2020, 9(1), 189; https://doi.org/10.3390/cells9010189
Received: 25 November 2019 / Revised: 31 December 2019 / Accepted: 9 January 2020 / Published: 11 January 2020
(This article belongs to the Special Issue Structure and Function of Healthy and Diseased Myelin)
Background: Myelin is an essential component of the peripheral and central nervous system, enabling fast axonal conduction and supporting axonal integrity; limited tools exist for analysis of myelin composition in-vivo. Objective: To demonstrate that the photophysical properties of myelin-incorporated solvatochromic dyes can be exploited to probe the biochemical composition of living peripheral nerve myelin at high spatial resolution. Methods: Using the myelin-incorporated fluorescent dye Nile Red we sequentially analyzed the spectral characteristics of remyelinating myelin membranes both in-vitro and in-vivo, including in living rats. Results: We demonstrated a consistent bi-phasic evolution of emission spectra during early remyelination, and visually report the reliable biochemical flux of myelin membrane composition in-vitro and in-vivo. Conclusions: Solvatochromic spectroscopy enables the analysis of myelin membrane maturity during remyelination, and can be performed in-vivo. As the formation of myelin during early-to-late remyelination likely incorporates fluctuating fractions of lipophilic components and changes in lateral membrane mobility, we propose that our spectrochemical data reflects the observation of these biochemical processes. View Full-Text
Keywords: intravital imaging; myelin; PNS; remyelination; spectral confocal microscopy; solvatochromism intravital imaging; myelin; PNS; remyelination; spectral confocal microscopy; solvatochromism
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Grochmal, J.; Teo, W.; Gambhir, H.; Kumar, R.; Stratton, J.A.; Dhaliwal, R.; Brideau, C.; Biernaskie, J.; Stys, P.; Midha, R. Spectral Characterization of Stem Cell-Derived Myelination within the Injured Adult PNS Using the Solvatochromic Dye Nile Red. Cells 2020, 9, 189.

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