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Open AccessArticle

Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa?

1
Laboratory of Reproductive Physiology, Institute of Animal Biochemistry and Genetics, Centre of Biosciences, Slovak Academy of Sciences, 840 05 Bratislava, Slovakia
2
Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 165 00 Prague 6, Czech Republic
3
Laboratory of Reproductive Biology, Institute of Biotechnology CAS, v.v.i., BIOCEV, 252 50 Vestec, Czech Republic
*
Author to whom correspondence should be addressed.
Cells 2020, 9(1), 183; https://doi.org/10.3390/cells9010183 (registering DOI)
Received: 22 November 2019 / Revised: 2 January 2020 / Accepted: 7 January 2020 / Published: 10 January 2020
Estrogens are steroid hormones that affect a wide range of physiological functions. The effect of estrogens on male reproductive tissues and sperm cells through specific receptors is essential for sperm development, maturation, and function. Although estrogen receptors (ERs) have been studied in several mammalian species, including humans, they have not yet been described in bull spermatozoa and reproductive tissues. In this study, we analyzed the presence of all types of ERs (ESR1, ESR2, and GPER1) in bull testicular and epididymal tissues and epididymal and ejaculated spermatozoa, and we characterize them here for the first time. We observed different localizations of each type of ER in the sperm head by immunofluorescent microscopy. Additionally, using a selected polyclonal antibody, we found that each type of ER in bull sperm extracts had two isoforms with different molecular masses. The detailed detection of ERs is a prerequisite not only for understanding the effect of estrogen on all reproductive events but also for further studying the negative effect of environmental estrogens (endocrine disruptors) on processes that lead to fertilization. View Full-Text
Keywords: reproduction; steroid hormones; testes; epididymis; bovine; plasma membrane reproduction; steroid hormones; testes; epididymis; bovine; plasma membrane
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MDPI and ACS Style

Antalikova, J.; Secova, P.; Horovska, L.; Krejcirova, R.; Simonik, O.; Jankovicova, J.; Bartokova, M.; Tumova, L.; Manaskova-Postlerova, P. Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa? Cells 2020, 9, 183.

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