Next Article in Journal
VAPB ER-Aggregates, A Possible New Biomarker in ALS Pathology
Next Article in Special Issue
Myxobacteria-Derived Outer Membrane Vesicles: Potential Applicability Against Intracellular Infections
Previous Article in Journal
In Silico Analysis of the Age-Dependent Evolution of the Transcriptome of Mouse Skin Stem Cells
Previous Article in Special Issue
Monitoring Therapy Efficiency in Cancer through Extracellular Vesicles
Open AccessArticle

Adipose Stem Cell-Derived Extracellular Vesicles Induce Proliferation of Schwann Cells via Internalization

Research Laboratory of the Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, 1090 Vienna, Austria
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Cells 2020, 9(1), 163; https://doi.org/10.3390/cells9010163
Received: 31 October 2019 / Revised: 13 December 2019 / Accepted: 19 December 2019 / Published: 9 January 2020
(This article belongs to the Special Issue Therapeutic Applications of Extracellular Vesicles)
Recent studies showed a beneficial effect of adipose stem cell-derived extracellular vesicles (ADSC-EVs) on sciatic nerve repair, presumably through Schwann cell (SC) modulation. However, it has not yet been elucidated whether ADSC-EVs exert this supportive effect on SCs by extracellular receptor binding, fusion to the SC membrane, or endocytosis mediated internalization. ADSCs, ADSC-EVs, and SCs were isolated from rats and characterized according to associated marker expression and properties. The proliferation rate of SCs in response to ADSC-EVs was determined using a multicolor immunofluorescence staining panel followed by automated image analysis. SCs treated with ADSC-EVs and silica beads were further investigated by 3-D high resolution confocal microscopy and live cell imaging. Our findings demonstrated that ADSC-EVs significantly enhanced the proliferation of SCs in a time- and dose-dependent manner. 3-D image analysis revealed a perinuclear location of ADSC-EVs and their accumulation in vesicular-like structures within the SC cytoplasm. Upon comparing intracellular localization patterns of silica beads and ADSC-EVs in SCs, we found striking resemblance in size and distribution. Live cell imaging visualized that the uptake of ADSC-EVs preferentially took place at the SC processes from which the EVs were transported towards the nucleus. This study provided first evidence for an endocytosis mediated internalization of ADSC-EVs by SCs and underlines the therapeutic potential of ADSC-EVs in future approaches for nerve regeneration. View Full-Text
Keywords: Schwann cells; adipose-derived stem cells; extracellular vesicles; endocytosis; proliferation; nerve regeneration; nerve injury Schwann cells; adipose-derived stem cells; extracellular vesicles; endocytosis; proliferation; nerve regeneration; nerve injury
Show Figures

Graphical abstract

MDPI and ACS Style

Haertinger, M.; Weiss, T.; Mann, A.; Tabi, A.; Brandel, V.; Radtke, C. Adipose Stem Cell-Derived Extracellular Vesicles Induce Proliferation of Schwann Cells via Internalization. Cells 2020, 9, 163.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop