Absence of Uncoupling Protein-3 at Thermoneutrality Impacts Lipid Handling and Energy Homeostasis in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Animals
2.3. Metabolic Parameters
2.4. SkM Mitochondrial Respiration
2.5. Separation of Respiratory Complexes by Blue-Native Page (BN-PAGE) and Histochemical Staining for in-Gel Activity
2.6. Determination of Glycerol Release from White Adipose Tissue
2.7. Hystological Analysis
2.8. Body Composition and Energy Gains
2.9. Statistical Analysis
3. Results
3.1. UCP3 Ablation Affects Resting Metabolic Rate, Energy Expenditure, and Fatty Acid Utilization in Adult Mice Acclimated at Thermoneutrality
3.2. Effect of UCP3 Ablation on Body Weight, Energy Efficiency, and Body Composition in Mice Fed Either with a Standard Low Fat Diet or a High Fat Diet
3.3. Effect of UCP3 Ablation on Visceral Adipose Tissue and Lipid Accumulation in Lean Tissue in Mice Fed Either with a Standard/Low Fat Diet or a High Fat Diet
4. Discussion
- (i)
- Mice habitual activity levels have not been evaluated. This is known to influence parameters, such as energy efficiency, body weight gain, and lipid accumulation. Thus, at the moment, it is unclear whether mouse activity levels were altered between gene and or diets, or whether activity levels contributed to study outcomes;
- (ii)
- Whole animal metabolic rate, respiratory quotient, mitochondrial functionality, and WAT lipolysis have only been evaluated in mice that were fed a standard diet. Thus, the estimation of the impact of high fat diet feeding on the above parameters would allow for better clarification of how they are influenced by different diet regimen and the eventual existence of an interaction diet-genotype;
- (iii)
- The evaluation of lipid serum levels, not detected in the present paper, would have provided additional knowledge regarding the role played by UCP3 in influencing lipid metabolism.
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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WT Time 0 | KO Time 0 | |
---|---|---|
Body weight (g) | 13.6± 0.9 | 11.5 ± 0.8 |
Body composition Water (%) | 65 ± 1.7 | 67 ± 1.5 |
Lipids (%) | 13.8 ± 0.3 | 12.7 ± 0.9 |
Proteins (%) | 16.5 ± 2.4 | 12.8 ± 2.6 |
Energy content/g of animal | 8.9 ± 0.5 | 7.64 ± 0.5 |
WT-WT | KO-STD | WT-HFD | KO-HFD | |
---|---|---|---|---|
Initial weight (g) | 11.88 ± 1.1 a | 10.3 ± 1.01 a | 14.50 ± 1.2 a | 11.14 ± 1.2 a |
Final weight (g) | 32.4 ± 1.1 a | 29.3 ± 1.1 b | 38.44 ± 0.6 c | 38.98 ± 1.2 c |
Body weight gain (g) | 19.0 ± 1.4 a | 19.0 ± 1.1 a | 23.94 ± 1.0 b | 27.79 ± 0.94 b |
Food intake (kJ) | 3294 ± 85 a | 3264 ± 107 a | 4107 ± 72 b | 3938 ± 96 b |
Body rough energy efficiency [Body weight gain (g)/food intake (MJ)] | 6.33 ± 0.35 a,b | 5.86 ± 0.24 a | 5.85 ± 0.29 a | 7.08 ± 0.29 b |
Visceral WAT (g) | 2.01 ± 0.14 a | 1.36 ± 0.08 b | 3.67 ± 0.16 c | 3.40 ± 0.24 c |
WAT weight/body weight * 100 | 6.09 ± 0.28 a | 4.62 ± 0.17 b | 9.51 ± 0.31 c | 9.09 ± 0.67 c |
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Lombardi, A.; Busiello, R.A.; De Matteis, R.; Lionetti, L.; Savarese, S.; Moreno, M.; Gentile, A.; Silvestri, E.; Senese, R.; de Lange, P.; et al. Absence of Uncoupling Protein-3 at Thermoneutrality Impacts Lipid Handling and Energy Homeostasis in Mice. Cells 2019, 8, 916. https://doi.org/10.3390/cells8080916
Lombardi A, Busiello RA, De Matteis R, Lionetti L, Savarese S, Moreno M, Gentile A, Silvestri E, Senese R, de Lange P, et al. Absence of Uncoupling Protein-3 at Thermoneutrality Impacts Lipid Handling and Energy Homeostasis in Mice. Cells. 2019; 8(8):916. https://doi.org/10.3390/cells8080916
Chicago/Turabian StyleLombardi, Assunta, Rosa Anna Busiello, Rita De Matteis, Lillà Lionetti, Sabrina Savarese, Maria Moreno, Alessandra Gentile, Elena Silvestri, Rosalba Senese, Pieter de Lange, and et al. 2019. "Absence of Uncoupling Protein-3 at Thermoneutrality Impacts Lipid Handling and Energy Homeostasis in Mice" Cells 8, no. 8: 916. https://doi.org/10.3390/cells8080916