IGFs Potentiate TAC3-induced SLα Expression via Upregulation of TACR3 Expression in Grass Carp Pituitary Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Reagents
2.2. Molecular Cloning, Tissue Expression, and Structural Analysis of Grass Carp TACR3
2.3. Functional Expression of Grass Carp TACR3 in HEK-293 Cells
2.4. Receptor Binding Assay for Grass Carp TACR3
2.5. Fluorescence Imaging of TACR3 Binding
2.6. RT-PCR for TACR3 and IGF-IR Expression in Immune-Identified Somatolactin (SL) Cells
2.7. Measurement of SLα and TACR3 mRNA Expression
2.8. Western Blot for Signaling Kinases
2.9. Data Transformation and Statistics
3. Results
3.1. Molecular Cloning and Tissue Distribution of Grass Carp TACR3
3.2. Functional Expression of Grass Carp TACR3
3.3. IGF-Induced TACR3 mRNA Expression in Grass Carp Pituitary Cells
Synergistic Effects of IGF and TAC3 Gene Products on SLα mRNA Expression
3.4. Signal Transduction for the Synergistic Effect of IGFs and TACR3 Activation
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
AC | Adenylyl cyclase |
Akt | protein kinase B |
CaM | Calmodulin |
CaMK-II | CaM-dependent protein kinase II |
DAG | diacylglycerol |
ERK | extracellular signal-regulated kinase |
FAM | fluorescein amidite |
GPCR | G protein coupled receptor |
Gq | G-protein alpha subunit, group Q |
IGF | insulin-like growth factor |
IP3 | Inositol 1,4,5-triphosphate |
LCM | laser capture microdissection |
MAPK | mitogen-activated protein kinase |
mTOR | mammalian target of rapamycin |
TACR3 | tachykinin receptor 3 |
NIL | neurointermediate lobe |
NKB | neurokinin B |
NKBRP | NKB-related peptide |
PACAP | pituitary adenylate cyclase activating peptide |
PI3K | phosphatidylinositol-3-kinase |
PKC | Protein kinase C |
PKA | Protein kinase A |
PLC | phospholipase C |
RLU | relative luminescence unit |
SL | Somatolactin |
SP | Substance P |
TAC3 | tachykinin 3 |
TACR3 | tachykinin receptor 3 |
VSCC | voltage sensitive calcium channel |
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Tachykinins | TAC1 | TAC3a | TAC3b | |||
---|---|---|---|---|---|---|
Luciferase Reporters | SP | NKA | NKBa | NKBRPa | NKBb | NKBRPb |
ED50 (nM) for NK3R-mediated luciferase activity | ||||||
CRE-Luc | 701.8 ± 50.1 | 1512.3 ± 25.1 | 26.9 ± 3.2 | 17.9 ± 2.5 | >10 µM | 15.9 ± 2.9 |
AP1-Luc | 954.1 ± 33.3 | 719.2 ± 39.3 | 36.5 ± 7.8 | 27.5 ± 5.3 | >10 µM | 27.1 ± 2.3 |
NFAT-Luc | 1071.3 ±65.4 | 1154.4 ± 30.8 | 37.1 ± 8.1 | 30.1 ± 2.2 | >10 µM | 23.9 ± 6.2 |
SRE-Luc | 1060.2 ± 34.8 | 752.8 ± 20.5 | 15.5 ± 1.5 | 22.3 ± 5.8 | >10 µM | 12.7 ± 3.8 |
ED50 (nM) for Ca2+ responses under Ca2+-containing/Ca2+-free condition | ||||||
Ca2+-free | 492.4 ± 48.2 | 480.8 ± 26.5 | 31.8 ± 3.6 | 38.6 ± 2.8 | >10 µM | 40.4 ± 7.8 |
Ca2+-containing | 487.1 ± 37.7 | 392.3 ± 23.4 | 50.3 ± 8.4 | 31.7 ± 4.9 | >10 µM | 33.2 ± 3.8 |
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Hu, G.; He, M.; Ko, W.K.W.; Ye, C.; Hu, Q.; Wong, A.O.L. IGFs Potentiate TAC3-induced SLα Expression via Upregulation of TACR3 Expression in Grass Carp Pituitary Cells. Cells 2019, 8, 887. https://doi.org/10.3390/cells8080887
Hu G, He M, Ko WKW, Ye C, Hu Q, Wong AOL. IGFs Potentiate TAC3-induced SLα Expression via Upregulation of TACR3 Expression in Grass Carp Pituitary Cells. Cells. 2019; 8(8):887. https://doi.org/10.3390/cells8080887
Chicago/Turabian StyleHu, Guangfu, Mulan He, Wendy K. W. Ko, Cheng Ye, Qiongyao Hu, and Anderson O. L. Wong. 2019. "IGFs Potentiate TAC3-induced SLα Expression via Upregulation of TACR3 Expression in Grass Carp Pituitary Cells" Cells 8, no. 8: 887. https://doi.org/10.3390/cells8080887