EGFR/ErbB Inhibition Promotes OPC Maturation up to Axon Engagement by Co-Regulating PIP2 and MBP
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Compound Treatments
2.3. Gene Silencing and Transfection
2.4. Crude Extract Preparation and Immunoblot Analysis
2.5. Quantitative Immunofluorescence Analysis and Confocal Microscopy
2.6. Evaluation of Cell Engagement on PS Microfibers
2.7. Total RNA Extraction and qPCR
2.8. Bioinformatics and Statistical Methods
3. Results
3.1. Gefitinib Promotes MBP Expression and Oli-neuM Differentiation in Myelinating OLs
3.2. Gefitinib and Clobetasol Treatment Upregulate RXRγ and Gli2 Expression
3.3. EGFR/ErbB Inhibition Promotes MBP Expression and Gefitinib or siEGFR Silencing Combined with Clobetasol Treatment Potently Stimulates MBP Expression and Oli-neuM Differentiation
3.4. Clobetasol and Gefitinib Co-Treatment Enhances Oli-neuM Differentiation by Co-Regulating PIP2 Availability with MBP Expression
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Nocita, E.; Del Giovane, A.; Tiberi, M.; Boccuni, L.; Fiorelli, D.; Sposato, C.; Romano, E.; Basoli, F.; Trombetta, M.; Rainer, A.; et al. EGFR/ErbB Inhibition Promotes OPC Maturation up to Axon Engagement by Co-Regulating PIP2 and MBP. Cells 2019, 8, 844. https://doi.org/10.3390/cells8080844
Nocita E, Del Giovane A, Tiberi M, Boccuni L, Fiorelli D, Sposato C, Romano E, Basoli F, Trombetta M, Rainer A, et al. EGFR/ErbB Inhibition Promotes OPC Maturation up to Axon Engagement by Co-Regulating PIP2 and MBP. Cells. 2019; 8(8):844. https://doi.org/10.3390/cells8080844
Chicago/Turabian StyleNocita, Emanuela, Alice Del Giovane, Marta Tiberi, Laura Boccuni, Denise Fiorelli, Carola Sposato, Elena Romano, Francesco Basoli, Marcella Trombetta, Alberto Rainer, and et al. 2019. "EGFR/ErbB Inhibition Promotes OPC Maturation up to Axon Engagement by Co-Regulating PIP2 and MBP" Cells 8, no. 8: 844. https://doi.org/10.3390/cells8080844