Cellular Plasmalogen Content Does Not Influence Arachidonic Acid Levels or Distribution in Macrophages: A Role for Cytosolic Phospholipase A2γ in Phospholipid Remodeling
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cell Culture
2.3. Quantitative PCR
2.4. Small Interfering RNA (siRNA) Transfection
2.5. Gas Chromatography/Mass Spectrometry (GC/MS) Analyses
2.6. Liquid Chromatography/Mass Spectrometry (LC/MS) Analyses of Phospholipids
2.7. Liquid Chromatography/Mass Spectrometry (LC/MS) Analyses of Eicosanoids
2.8. Measurement of Phospholipid Arachidonate Remodeling
2.9. Statistical Analysis
3. Results
3.1. AA Distribution in RAW264.7 Cells and Plasmalogen-Deficient Variants
3.2. Importance of Plasmalogen Content for Phosphospholipid AA Remodeling
3.3. Role of Plasmalogens in Functional Responses of Macrophages to Receptor Stimulation
3.4. Studies Utilizing AA-enriched Cells
3.5. Phospholipase A2 Inhibition Studies
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Lebrero, P.; Astudillo, A.M.; Rubio, J.M.; Fernández-Caballero, L.; Kokotos, G.; Balboa, M.A.; Balsinde, J. Cellular Plasmalogen Content Does Not Influence Arachidonic Acid Levels or Distribution in Macrophages: A Role for Cytosolic Phospholipase A2γ in Phospholipid Remodeling. Cells 2019, 8, 799. https://doi.org/10.3390/cells8080799
Lebrero P, Astudillo AM, Rubio JM, Fernández-Caballero L, Kokotos G, Balboa MA, Balsinde J. Cellular Plasmalogen Content Does Not Influence Arachidonic Acid Levels or Distribution in Macrophages: A Role for Cytosolic Phospholipase A2γ in Phospholipid Remodeling. Cells. 2019; 8(8):799. https://doi.org/10.3390/cells8080799
Chicago/Turabian StyleLebrero, Patricia, Alma M. Astudillo, Julio M. Rubio, Lidia Fernández-Caballero, George Kokotos, María A. Balboa, and Jesús Balsinde. 2019. "Cellular Plasmalogen Content Does Not Influence Arachidonic Acid Levels or Distribution in Macrophages: A Role for Cytosolic Phospholipase A2γ in Phospholipid Remodeling" Cells 8, no. 8: 799. https://doi.org/10.3390/cells8080799