Next Article in Journal
The Intrinsic GDP/GTP Exchange Activities of Cdc42 and Rac1 Are Critical Determinants for Their Specific Effects on Mobilization of the Actin Filament System
Previous Article in Journal
The IL4I1 Enzyme: A New Player in the Immunosuppressive Tumor Microenvironment
Open AccessArticle

Copine A Interacts with Actin Filaments and Plays a Role in Chemotaxis and Adhesion

Department of Biology, Central Michigan University, Mount Pleasant, MI 48859, USA
Department of Pharmacology and Cancer, Duke University Medical Center, Durham, NC 27708, USA
Author to whom correspondence should be addressed.
Cells 2019, 8(7), 758;
Received: 25 May 2019 / Revised: 17 July 2019 / Accepted: 19 July 2019 / Published: 21 July 2019
(This article belongs to the Section Cell Motility and Adhesion)
Copines make up a family of calcium-dependent, phospholipid-binding proteins found in numerous eukaryotic organisms. Copine proteins consist of two C2 domains at the N-terminus followed by an A domain similar to the von Willebrand A domain found in integrins. We are studying copine protein function in the model organism, Dictyostelium discoideum, which has six copine genes, cpnA-cpnF. Previous research showed that cells lacking the cpnA gene exhibited a cytokinesis defect, a contractile vacuole defect, and developmental defects. To provide insight into the role of CpnA in these cellular processes, we used column chromatography and immunoprecipitation to isolate proteins that bind to CpnA. These proteins were identified by mass spectrometry. One of the proteins identified was actin. Purified CpnA was shown to bind to actin filaments in a calcium-dependent manner in vitro. cpnA cells exhibited defects in three actin-based processes: chemotaxis, cell polarity, and adhesion. These results suggest that CpnA plays a role in chemotaxis and adhesion and may do so by interacting with actin filaments. View Full-Text
Keywords: copine; actin; calcium; Dictyostelium; cAMP; chemotaxis; adhesion copine; actin; calcium; Dictyostelium; cAMP; chemotaxis; adhesion
Show Figures

Figure 1

MDPI and ACS Style

Buccilli, M.J.; Ilacqua, A.N.; Han, M.; Banas, A.A.; Wight, E.M.; Mao, H.; Perry, S.P.; Salter, T.S.; Loiselle, D.R.; Haystead, T.A.; Damer, C.K. Copine A Interacts with Actin Filaments and Plays a Role in Chemotaxis and Adhesion. Cells 2019, 8, 758.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop