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Open AccessArticle

Distinct Protein Expression Networks are Activated in Microglia Cells after Stimulation with IFN-γ and IL-4

Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, Italy
Division of Neuroscience, Institute of Experimental Neurology—IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy
Institute of Food Production Sciences, C.N.R. Unit of Lecce, 73100 Lecce, Italy
U1192 INSERM, Laboratoire PRISM: Protéomique, Réponse Inflammatoire, Spectrométrie de Masse, Université de Lille, F-59000 Lille, France
Authors to whom correspondence should be addressed.
These authors contributed equally to this manuscript.
These authors shared senior authorship.
Cells 2019, 8(6), 580;
Received: 8 May 2019 / Revised: 30 May 2019 / Accepted: 10 June 2019 / Published: 12 June 2019
(This article belongs to the Special Issue Microglia in Neurodegenerative Diseases)
Microglia cells are the primary immune population of the central nervous system with a role in the regulation of several physiological and pathological conditions. Upon appropriate stimulation, microglia cells can be polarized in a pro-inflammatory M1-like or anti-inflammatory M2-like status. Biological processes and pathways engaged in microglia polarization are starting to be elucidated. To help clarify this, we used a liquid chromatography-mass spectrometry (LC-MS/MS) label free approach to characterize the proteomic profile of human microglia cell line (CHME-5) stimulated with gamma-interferon (IFN-γ) and interleukin-4 (IL-4) to induce a M1 or M2 phenotype, respectively. Outside the classical M1/M2 polarization markers, the M1 status appears to center around the activation of a classical inflammatory response and through the activation of multiple signaling pathways. M2 polarization resulted in a different pattern of protein modulation related to RNA and cellular metabolic processes. Together, our findings provide information regarding the protein changes specific to M1 and M2 activation states, and potentially link the polarization of microglia cells to the acquisition of a specific proteomic profile. View Full-Text
Keywords: microglia plasticity; microglia; IFN-γ; IL-4; mass spectrometry; proteomics microglia plasticity; microglia; IFN-γ; IL-4; mass spectrometry; proteomics
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Vergara, D.; Nigro, A.; Romano, A.; De Domenico, S.; Damato, M.; Franck, J.; Coricciati, C.; Wistorski, M.; Cardon, T.; Fournier, I.; Quattrini, A.; Salzet, M.; Furlan, R.; Maffia, M. Distinct Protein Expression Networks are Activated in Microglia Cells after Stimulation with IFN-γ and IL-4. Cells 2019, 8, 580.

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