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Quantitative Analysis of Nuclear Lamins Imaged by Super-Resolution Light Microscopy

1
Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
2
Department of Biophysics, UT Southwestern Medical Center, Dallas, TX 75390, USA
3
Institute of Biomedicine, Research Center for Cancer, Infections and Immunity, University of Turku, 20520 Turku, Finland
4
Department of Pathology, Turku University Hospital, 20520 Turku, Finland
*
Authors to whom correspondence should be addressed.
Cells 2019, 8(4), 361; https://doi.org/10.3390/cells8040361
Received: 21 March 2019 / Revised: 13 April 2019 / Accepted: 14 April 2019 / Published: 18 April 2019
(This article belongs to the Special Issue Development and Challenges in Microscopy for Cellular Imaging)
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Abstract

The nuclear lamina consists of a dense fibrous meshwork of nuclear lamins, Type V intermediate filaments, and is ~14 nm thick according to recent cryo-electron tomography studies. Recent advances in light microscopy have extended the resolution to a scale allowing for the fine structure of the lamina to be imaged in the context of the whole nucleus. We review quantitative approaches to analyze the imaging data of the nuclear lamina as acquired by structured illumination microscopy (SIM) and single molecule localization microscopy (SMLM), as well as the requisite cell preparation techniques. In particular, we discuss the application of steerable filters and graph-based methods to segment the structure of the four mammalian lamin isoforms (A, C, B1, and B2) and extract quantitative information. View Full-Text
Keywords: lamins; structured illumination microscopy; single molecule localization microscopy; steerable filters; computational geometry; delaunay triangulation; voronoi tessellation lamins; structured illumination microscopy; single molecule localization microscopy; steerable filters; computational geometry; delaunay triangulation; voronoi tessellation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Kittisopikul, M.; Virtanen, L.; Taimen, P.; Goldman, R.D. Quantitative Analysis of Nuclear Lamins Imaged by Super-Resolution Light Microscopy. Cells 2019, 8, 361.

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